2X7L
Implications of the HIV-1 Rev dimer structure at 3.2A resolution for multimeric binding to the Rev response element
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 87.678, 87.692, 176.334 |
| Unit cell angles | 94.86, 95.50, 104.60 |
Refinement procedure
| Resolution | 48.780 - 3.170 |
| R-factor | 0.2346 |
| Rwork | 0.234 |
| R-free | 0.25000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 1JPS 2qr0 2r8s 3bdy 1tzh 1za3 2R0K 2V7N 3dvg 1b2w 1bey 1tzi 2fjf 2qqn 1b4j 2fgw 2fjh 3d85 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.320 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.9.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.800 | 3.260 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.120 | 1.000 |
| Number of reflections | 83664 | |
| <I/σ(I)> | 9.9 | 1.1 |
| Completeness [%] | 99.0 | 98.8 |
| Redundancy | 3.4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 12% POLYETHYLENE GLYCOL (PEG) 6000, 100 MM DI-AMMONIUM PHOSPHATE (DAP), AND 100 MM TRIS/HCL (PH 8.5) |
