3D85

Crystal structure of IL-23 in complex with neutralizing FAB

Summary for 3D85

• Entry DOI: 10.2210/pdb3d85/pdb
• Related: 1F45 3D87
• Descriptor: FAB of antibody 7G10, light chain, FAB of antibody 7G10, heavy chain, Interleukin-23 subunit p19, ... (7 entities in total)
• Functional Keywords: interleukin-23, fab, immune system-cytokine complex, immune system/cytokine
• Biological source: Homo sapiens (human); More
• Cellular location: Secreted: Q9NPF7 P29460
• Total number of polymer chains: 4
• Total formula weight: 102027.23

Authors

Beyer, B.M.,Ingram, R.,Ramanathan, L.,Reichert, P.,Le, H.,Madison, V. (deposition date: 2008-05-22, release date: 2008-09-02, Last modification date: 2024-11-20)

Primary citation

Beyer, B.M.,Ingram, R.,Ramanathan, L.,Reichert, P.,Le, H.V.,Madison, V.,Orth, P.
Crystal structures of the pro-inflammatory cytokine interleukin-23 and its complex with a high-affinity neutralizing antibody
J.Mol.Biol., 382:942-955, 2008

PubMed Abstract: Interleukin (IL)-23 is a pro-inflammatory cytokine playing a key role in the pathogenesis of several autoimmune and inflammatory diseases. We have determined the crystal structures of the heterodimeric p19-p40 IL-23 and its complex with the Fab (antigen-binding fragment) of a neutralizing antibody at 2.9 and 1.9 A, respectively. The IL-23 structure closely resembles that of IL-12. They share the common p40 subunit, and IL-23 p19 overlaps well with IL-12 p35. Along the hydrophilic heterodimeric interface, fewer charged residues are involved for IL-23 compared with IL-12. The binding site of the Fab is located exclusively on the p19 subunit, and comparison with published cytokine-receptor structures suggests that it overlaps with the IL-23 receptor binding site.

PubMed: 18708069
DOI: 10.1016/j.jmb.2008.08.001

Experimental method

X-RAY DIFFRACTION (1.9 Å)