3D85

Crystal structure of IL-23 in complex with neutralizing FAB

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	FAB of antibody 7G10, light chain	polymer	214	23455.0	1		Homo sapiens (human)
2	B (B)	FAB of antibody 7G10, heavy chain	polymer	223	23843.7	1	Pfam (PF07654) UniProt (by SIFTS) (P01857)	Homo sapiens (human)
3	C (C)	Interleukin-23 subunit p19	polymer	178	19669.1	1	UniProt (Q9NPF7) Pfam (PF16649)	Homo sapiens (human)	Interleukin-23 subunit alpha; IL-23 subunit alpha; IL-23p19
4	D (D)	Interleukin-12 subunit p40	polymer	306	34754.0	1	UniProt (P29460) Pfam (PF10420)	Homo sapiens (human)	IL-12B; IL-12 subunit p40; Cytotoxic lymphocyte maturation factor 40 kDa subunit; CLMF p40; NK cell stimulatory factor chain 2; NKSF2
5	E (A)	IMIDAZOLE	non-polymer		69.1	1	Chemie (IMD)
6	F, G (A, B)	(4S)-2-METHYL-2,4-PENTANEDIOL	non-polymer		118.2	2	Chemie (MPD)
7	H, I, J, K (A, B, C, D)	water	water		18.0	906	Chemie (HOH)

Sequence modifications

C: 1 - 170 (UniProt: Q9NPF7)

PDB	External Database	Details
Gly 171	-	expression tag
Ser 172	-	expression tag
His 173	-	expression tag
His 174	-	expression tag
His 175	-	expression tag
His 176	-	expression tag
His 177	-	expression tag
His 178	-	expression tag

D: 1 - 306 (UniProt: P29460)

PDB	External Database	Details
Gln 200	Asn 222	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	101721.8
Non-Polymers*	Number of molecules	3
	Total formula weight	305.4
All*	Total formula weight	102027.2

*Water molecules are not included.