3D85
Crystal structure of IL-23 in complex with neutralizing FAB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-24 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 75.386, 62.091, 107.261 |
| Unit cell angles | 90.00, 97.44, 90.00 |
Refinement procedure
| Resolution | 106.600 - 1.900 |
| R-factor | 0.1721 |
| Rwork | 0.171 |
| R-free | 0.21450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ngy |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.254 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | BUSTER-TNT (2.1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 106.600 | 1.960 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.082 | 0.466 |
| Number of reflections | 77159 | |
| <I/σ(I)> | 15.6 | 3.1 |
| Completeness [%] | 99.6 | 99.6 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | 10% MPD, 7% PEG 6000, 100mM Imidazole, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
