7Z4H
| SpCas9 bound to 14-nucleotide complementary DNA substrate | Descriptor: | CRISPR-associated endonuclease Cas9/Csn1, Non-target strand of 14-nucleotide complementary DNA substrate, Target strand of 14-nucleotide complementary DNA substrate, ... | Authors: | Pacesa, M, Jinek, M. | Deposit date: | 2022-03-03 | Release date: | 2022-08-31 | Last modified: | 2022-09-14 | Method: | ELECTRON MICROSCOPY (3.49 Å) | Cite: | R-loop formation and conformational activation mechanisms of Cas9. Nature, 609, 2022
|
|
7Z4J
| |
5FW3
| Crystal structure of SpCas9 variant VRER bound to sgRNA and TGCG PAM target DNA | Descriptor: | CRISPR-ASSOCIATED ENDONUCLEASE CAS9/CSN1, MAGNESIUM ION, NON-TARGET DNA STRAND, ... | Authors: | Anders, C, Bargsten, K, Jinek, M. | Deposit date: | 2016-02-11 | Release date: | 2016-06-15 | Last modified: | 2024-01-10 | Method: | X-RAY DIFFRACTION (2.7 Å) | Cite: | Structural Plasticity of Pam Recognition by Engineered Variants of the RNA-Guided Endonuclease Cas9. Mol.Cell, 61, 2016
|
|
5FW1
| Crystal structure of SpyCas9 variant VQR bound to sgRNA and TGAG PAM target DNA | Descriptor: | CRISPR-ASSOCIATED ENDONUCLEASE CAS9/CSN1, MAGNESIUM ION, NON-TARGET DNA STRAND, ... | Authors: | Anders, C, Bargsten, K, Jinek, M. | Deposit date: | 2016-02-11 | Release date: | 2016-04-20 | Last modified: | 2024-01-10 | Method: | X-RAY DIFFRACTION (2.499 Å) | Cite: | Structural Plasticity of Pam Recognition by Engineered Variants of the RNA-Guided Endonuclease Cas9. Mol.Cell, 61, 2016
|
|
5FSH
| |
6O16
| Crystal structure of murine DHX37 in complex with RNA | Descriptor: | DEAH (Asp-Glu-Ala-His) box polypeptide 37, RNA (5'-R(*UP*UP*UP*UP*UP*UP*UP*UP*UP*U)-3') | Authors: | Boneberg, F, Brandmann, T, Kobel, L, van den Heuvel, J, Bargsten, K, Bammert, L, Kutay, U, Jinek, M. | Deposit date: | 2019-02-18 | Release date: | 2019-04-17 | Last modified: | 2024-03-13 | Method: | X-RAY DIFFRACTION (2.875 Å) | Cite: | Molecular mechanism of the RNA helicase DHX37 and its activation by UTP14A in ribosome biogenesis. Rna, 25, 2019
|
|
8QLO
| CryoEM structure of the apo SPARTA (BabAgo/TIR-APAZ) complex | Descriptor: | Short prokaryotic Argonaute, Toll/interleukin-1 receptor domain-containing protein | Authors: | Finocchio, G, Koopal, B, Potocnik, A, Heijstek, C, Jinek, M, Swarts, D. | Deposit date: | 2023-09-20 | Release date: | 2024-01-31 | Last modified: | 2024-03-06 | Method: | ELECTRON MICROSCOPY (2.57 Å) | Cite: | Target DNA-dependent activation mechanism of the prokaryotic immune system SPARTA. Nucleic Acids Res., 52, 2024
|
|
8QLP
| CryoEM structure of the RNA/DNA bound SPARTA (BabAgo/TIR-APAZ) tetrameric complex | Descriptor: | DNA (5'-D(*AP*CP*TP*AP*AP*TP*AP*GP*AP*TP*TP*AP*GP*AP*GP*CP*CP*GP*TP*C)-3'), MAGNESIUM ION, RNA (5'-R(*AP*UP*GP*AP*CP*GP*GP*CP*UP*CP*UP*AP*AP*UP*CP*UP*AP*UP*UP*AP*GP*U)-3'), ... | Authors: | Finocchio, G, Koopal, B, Potocnik, A, Heijstek, C, Jinek, M, Swarts, D. | Deposit date: | 2023-09-20 | Release date: | 2024-01-31 | Last modified: | 2024-03-06 | Method: | ELECTRON MICROSCOPY (3.14 Å) | Cite: | Target DNA-dependent activation mechanism of the prokaryotic immune system SPARTA. Nucleic Acids Res., 52, 2024
|
|
6QZK
| Structure of Clostridium butyricum Argonaute bound to a guide DNA (5' deoxycytidine) and a 19-mer target DNA | Descriptor: | Clostridium butyricum Argonaute, DNA target (5'-D(T*AP*TP*AP*CP*AP*AP*CP*CP*TP*AP*CP*TP*AP*CP*CP*TP*CP*T)-3'), FORMIC ACID, ... | Authors: | Swarts, D.C, Jinek, M, Hegge, J.W, Van der Oost, J. | Deposit date: | 2019-03-11 | Release date: | 2019-04-24 | Last modified: | 2024-01-24 | Method: | X-RAY DIFFRACTION (3.548 Å) | Cite: | DNA-guided DNA cleavage at moderate temperatures by Clostridium butyricum Argonaute. Nucleic Acids Res., 47, 2019
|
|
5AF2
| |
7ZYH
| Crystal structure of human CPSF30 in complex with hFip1 | Descriptor: | Cleavage and polyadenylation specificity factor subunit 4, Isoform 4 of Pre-mRNA 3'-end-processing factor FIP1, ZINC ION | Authors: | Muckenfuss, L.M, Jinek, M, Migenda Herranz, A.C, Clerici, M. | Deposit date: | 2022-05-24 | Release date: | 2022-09-14 | Last modified: | 2022-09-21 | Method: | X-RAY DIFFRACTION (2.2 Å) | Cite: | Fip1 is a multivalent interaction scaffold for processing factors in human mRNA 3' end biogenesis. Elife, 11, 2022
|
|
4UN4
| Crystal structure of Cas9 bound to PAM-containing DNA target with mismatches at positions 1-2 | Descriptor: | CRISPR-ASSOCIATED ENDONUCLEASE CAS9/CSN1, MAGNESIUM ION, NON-TARGET DNA STRAND, ... | Authors: | Anders, C, Niewoehner, O, Duerst, A, Jinek, M. | Deposit date: | 2014-05-25 | Release date: | 2014-07-23 | Last modified: | 2024-01-10 | Method: | X-RAY DIFFRACTION (2.371 Å) | Cite: | Structural Basis of Pam-Dependent Target DNA Recognition by the Cas9 Endonuclease Nature, 513, 2014
|
|
4UN5
| Crystal structure of Cas9 bound to PAM-containing DNA target containing mismatches at positions 1-3 | Descriptor: | CRISPR-ASSOCIATED ENDONUCLEASE CAS9/CSN1, MAGNESIUM ION, NON-TARGET DNA STRAND, ... | Authors: | Anders, C, Niewoehner, O, Duerst, A, Jinek, M. | Deposit date: | 2014-05-25 | Release date: | 2014-07-23 | Last modified: | 2024-01-10 | Method: | X-RAY DIFFRACTION (2.4 Å) | Cite: | Structural Basis of Pam-Dependent Target DNA Recognition by the Cas9 Endonuclease Nature, 513, 2014
|
|
4UN3
| Crystal structure of Cas9 bound to PAM-containing DNA target | Descriptor: | CRISPR-ASSOCIATED ENDONUCLEASE CAS9/CSN1, MAGNESIUM ION, NON-TARGET DNA STRAND, ... | Authors: | Anders, C, Niewoehner, O, Duerst, A, Jinek, M. | Deposit date: | 2014-05-25 | Release date: | 2014-07-23 | Last modified: | 2024-05-08 | Method: | X-RAY DIFFRACTION (2.593 Å) | Cite: | Structural Basis of Pam-Dependent Target DNA Recognition by the Cas9 Endonuclease Nature, 513, 2014
|
|
8BD5
| Cas12k-sgRNA-dsDNA-S15-TniQ-TnsC transposon recruitment complex | Descriptor: | 30S ribosomal protein S15, ADENOSINE-5'-TRIPHOSPHATE, DNA non-target strand, ... | Authors: | Schmitz, M, Querques, I, Oberli, S, Chanez, C, Jinek, M. | Deposit date: | 2022-10-18 | Release date: | 2022-12-28 | Last modified: | 2023-02-01 | Method: | ELECTRON MICROSCOPY (3.3 Å) | Cite: | Structural basis for the assembly of the type V CRISPR-associated transposon complex. Cell, 185, 2022
|
|
8BD4
| TniQ-capped Tns-ATP-dsDNA complex | Descriptor: | ADENOSINE-5'-TRIPHOSPHATE, DNA (5'-D(P*GP*AP*TP*CP*GP*AP*TP*CP*GP*AP*TP*CP*GP*AP*TP*C)-3'), MAGNESIUM ION, ... | Authors: | Querques, I, Schmitz, M, Oberli, S, Chanez, C, Jinek, M. | Deposit date: | 2022-10-18 | Release date: | 2022-12-28 | Last modified: | 2023-01-04 | Method: | ELECTRON MICROSCOPY (3.44 Å) | Cite: | Structural basis for the assembly of the type V CRISPR-associated transposon complex. Cell, 185, 2022
|
|
7ZO1
| SpCas9 bound to CD34 off-target9 DNA substrate | Descriptor: | CD34 off-target9 DNA non-target strand, CD34 off-target9 DNA target strand, CD34 sgRNA, ... | Authors: | Pacesa, M, Jinek, M. | Deposit date: | 2022-04-23 | Release date: | 2022-10-26 | Last modified: | 2024-01-31 | Method: | X-RAY DIFFRACTION (2.4 Å) | Cite: | Structural basis for Cas9 off-target activity. Cell, 185, 2022
|
|
8BD6
| Cas12k-sgRNA-dsDNA-TnsC non-productive complex. | Descriptor: | ADENOSINE-5'-TRIPHOSPHATE, Cas12k, DNA, ... | Authors: | Schmitz, M, Querques, I, Oberli, S, Chanez, C, Jinek, M. | Deposit date: | 2022-10-18 | Release date: | 2022-12-28 | Last modified: | 2023-07-26 | Method: | ELECTRON MICROSCOPY (4.1 Å) | Cite: | Structural basis for the assembly of the type V CRISPR-associated transposon complex. Cell, 185, 2022
|
|
5L6D
| |
5L6E
| |
7ZY4
| |
7OXA
| Target-bound SpCas9 complex with AAVS1 chimeric RNA-DNA guide | Descriptor: | AAVS1 non-target DNA strand, AAVS1 target DNA strand, CRISPR-associated endonuclease Cas9/Csn1, ... | Authors: | Donohoue, P, Pacesa, M, Lau, E, Vidal, B, Irby, M.J, Nyer, D.B, Rotstein, T, Banh, L, Toh, M.T, Gibson, J, Kohrs, B, Baek, K, Owen, A.L.G, Slorach, E.M, van Overbeek, M, Fuller, C.K, May, A.P, Jinek, M, Cameron, P. | Deposit date: | 2021-06-22 | Release date: | 2021-09-15 | Last modified: | 2024-01-31 | Method: | X-RAY DIFFRACTION (2.15 Å) | Cite: | Conformational control of Cas9 by CRISPR hybrid RNA-DNA guides mitigates off-target activity in T cells. Mol.Cell, 81, 2021
|
|
7OX7
| Target-bound SpCas9 complex with TRAC chimeric RNA-DNA guide | Descriptor: | CRISPR-associated endonuclease Cas9/Csn1, MAGNESIUM ION, POTASSIUM ION, ... | Authors: | Donohoue, P, Pacesa, M, Lau, E, Vidal, B, Irby, M.J, Nyer, D.B, Rotstein, T, Banh, L, Toh, M.T, Gibson, J, Kohrs, B, Baek, K, Owen, A.L.G, Slorach, E.M, van Overbeek, M, Fuller, C.K, May, A.P, Jinek, M, Cameron, P. | Deposit date: | 2021-06-22 | Release date: | 2021-09-15 | Last modified: | 2024-01-31 | Method: | X-RAY DIFFRACTION (2.6 Å) | Cite: | Conformational control of Cas9 by CRISPR hybrid RNA-DNA guides mitigates off-target activity in T cells. Mol.Cell, 81, 2021
|
|
7OX8
| Target-bound SpCas9 complex with TRAC full RNA guide | Descriptor: | CRISPR-associated endonuclease Cas9/Csn1, MAGNESIUM ION, POTASSIUM ION, ... | Authors: | Donohoue, P, Pacesa, M, Lau, E, Vidal, B, Irby, M.J, Nyer, D.B, Rotstein, T, Banh, L, Toh, M.T, Gibson, J, Kohrs, B, Baek, K, Owen, A.L.G, Slorach, E.M, van Overbeek, M, Fuller, C.K, May, A.P, Jinek, M, Cameron, P. | Deposit date: | 2021-06-22 | Release date: | 2021-09-15 | Last modified: | 2024-01-31 | Method: | X-RAY DIFFRACTION (2.75 Å) | Cite: | Conformational control of Cas9 by CRISPR hybrid RNA-DNA guides mitigates off-target activity in T cells. Mol.Cell, 81, 2021
|
|
7OX9
| Target-bound SpCas9 complex with AAVS1 all-RNA guide | Descriptor: | AAVS1 non-target DNA strand, AAVS1 target DNA strand, CRISPR-associated endonuclease Cas9/Csn1, ... | Authors: | Pacesa, M, Donohoue, P, May, A.P, Jinek, M, Cameron, P. | Deposit date: | 2021-06-22 | Release date: | 2021-09-15 | Last modified: | 2024-01-31 | Method: | X-RAY DIFFRACTION (2.45 Å) | Cite: | Conformational control of Cas9 by CRISPR hybrid RNA-DNA guides mitigates off-target activity in T cells. Mol.Cell, 81, 2021
|
|