7Z4J
SpCas9 bound to 18-nucleotide complementary DNA substrate in the catalytic state
Summary for 7Z4J
| Entry DOI | 10.2210/pdb7z4j/pdb |
| EMDB information | 14499 |
| Descriptor | sgRNA, Target strand of 18-nucleotide complementary DNA substrate, PAM-proximal end, Target strand of 18-nucleotide complementary DNA substrate, PAM-distal end, ... (7 entities in total) |
| Functional Keywords | crispr, cas9, r-loop, substrate binding, off-target, hydrolase |
| Biological source | Streptococcus pyogenes More |
| Total number of polymer chains | 5 |
| Total formula weight | 219118.33 |
| Authors | Pacesa, M.,Jinek, M. (deposition date: 2022-03-03, release date: 2022-08-31, Last modification date: 2022-09-14) |
| Primary citation | Pacesa, M.,Loeff, L.,Querques, I.,Muckenfuss, L.M.,Sawicka, M.,Jinek, M. R-loop formation and conformational activation mechanisms of Cas9. Nature, 609:191-196, 2022 Cited by PubMed: 36002571DOI: 10.1038/s41586-022-05114-0 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (2.99 Å) |
Structure validation
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