7Z4J
SpCas9 bound to 18-nucleotide complementary DNA substrate in the catalytic state
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | sgRNA | polymer | 102 | 33005.6 | 1 | synthetic construct | ||
| 2 | B (c) | Target strand of 18-nucleotide complementary DNA substrate, PAM-proximal end | polymer | 13 | 3967.6 | 1 | synthetic construct | ||
| 3 | C (C) | Target strand of 18-nucleotide complementary DNA substrate, PAM-distal end | polymer | 32 | 9780.3 | 1 | synthetic construct | ||
| 4 | D (D) | Non-target strand of 18-nucleotide complementary DNA substrate | polymer | 44 | 13567.8 | 1 | synthetic construct | ||
| 5 | E (B) | CRISPR-associated endonuclease Cas9/Csn1 | polymer | 1368 | 158699.8 | 1 | UniProt (Q99ZW2) Pfam (PF16593) Pfam (PF16592) Pfam (PF13395) Pfam (PF22702) Pfam (PF16595) | Streptococcus pyogenes | SpCas9,SpyCas9 |
| 6 | F, G, H, I (A, B) | MAGNESIUM ION | non-polymer | 24.3 | 4 | Chemie (MG) | |||
| 7 | J, K, L (A, C, B) | water | water | 18.0 | 8 | Chemie (HOH) |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 219021.1 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 97.2 | |
| All* | Total formula weight | 219118.3 |
