4UN4

Crystal structure of Cas9 bound to PAM-containing DNA target with mismatches at positions 1-2

Summary for 4UN4

Entry DOI	10.2210/pdb4un4/pdb
Related	4UN3 4UN5
Descriptor	SGRNA, CRISPR-ASSOCIATED ENDONUCLEASE CAS9/CSN1, TARGET DNA STRAND PROXIMAL FRAGMENT, ... (7 entities in total)
Functional Keywords	hydrolase-dna-rna complex, genome editing, rnp, protein-rna complex, hydrolase/dna/rna
Biological source	STREPTOCOCCUS PYOGENES More
Total number of polymer chains	5
Total formula weight	197779.31
Authors	Anders, C.,Niewoehner, O.,Duerst, A.,Jinek, M. (deposition date: 2014-05-25, release date: 2014-07-23, Last modification date: 2024-01-10)
Primary citation	Anders, C.,Niewoehner, O.,Duerst, A.,Jinek, M. Structural Basis of Pam-Dependent Target DNA Recognition by the Cas9 Endonuclease Nature, 513:569-, 2014 Cited by PubMed: 25079318 DOI: 10.1038/NATURE13579 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2.371 Å)

Structure validation