4V5L

The structure of EF-Tu and aminoacyl-tRNA bound to the 70S ribosome with a GTP analog

これはPDB形式変換不可エントリーです。

4V5L の概要

• エントリーDOI: 10.2210/pdb4v5l/pdb
• 関連するPDBエントリー: 1AB3 1AN7 1CQM 1CQN 1DK1 1EG0 1EMW 1F7Y 1FJG 1FKA 1GIX 1HNW 1HNX 1HNZ 1HR0 1I94 1I95 1I96 1I97 1IBK 1IBL 1IBM 1J5E 1JGO 1JGP 1JGQ 1KUQ 1L1U 1LOU 1MVR 1N32 1N33 1N34 1N36 1PNS 1PNX 1QD7 1QJH 1QKF 1QKH 1RIS 1TWT 1VOX 1VOZ 1XMO 1XMQ 1XNQ 1XNR 1YL4 2B64 2B9M 2B9O 2BVZ 2BXJ 2C77 2F4V 2J00 2J02 2JL5 2JL7 2UU9 2UUA 2UUB 2UUC 2UXB 2UXC 2UXD 2V46 2V48 2VQE 2VQF 2WDG 2WDH 2WDK 2WDM 2WH1 2WH3 2WRN 2WRQ 2X9R 2X9T 2XFZ 2XG1
• 分子名称: 16S RRNA, 30S RIBOSOMAL PROTEIN S10, 30S RIBOSOMAL PROTEIN S11, ... (63 entities in total)
• 機能のキーワード: ribosome, ef-tu, trna, gdpcp, gtpase
• 由来する生物種: THERMUS THERMOPHILUS; 詳細
• タンパク質・核酸の鎖数: 59
• 化学式量合計: 2325014.04

構造登録者

Voorhees, R.M.,Schmeing, T.M.,Ramakrishnan, V. (登録日: 2010-09-02, 公開日: 2014-07-09, 最終更新日: 2024-10-23)

主引用文献

Voorhees, R.M.,Schmeing, T.M.,Kelley, A.C.,Ramakrishnan, V.
The Mechanism for Activation of GTP Hydrolysis on the Ribosome.
Science, 330:835-, 2010

PubMed Abstract: Protein synthesis requires several guanosine triphosphatase (GTPase) factors, including elongation factor Tu (EF-Tu), which delivers aminoacyl-transfer RNAs (tRNAs) to the ribosome. To understand how the ribosome triggers GTP hydrolysis in translational GTPases, we have determined the crystal structure of EF-Tu and aminoacyl-tRNA bound to the ribosome with a GTP analog, to 3.2 angstrom resolution. EF-Tu is in its active conformation, the switch I loop is ordered, and the catalytic histidine is coordinating the nucleophilic water in position for inline attack on the γ-phosphate of GTP. This activated conformation is due to a critical and conserved interaction of the histidine with A2662 of the sarcin-ricin loop of the 23S ribosomal RNA. The structure suggests a universal mechanism for GTPase activation and hydrolysis in translational GTPases on the ribosome.

PubMed: 21051640
DOI: 10.1126/SCIENCE.1194460

実験手法

X-RAY DIFFRACTION (3.1 Å)