1XMO
Crystal Structure of mnm5U34t6A37-tRNALysUUU Complexed with AAG-mRNA in the Decoding Center
Summary for 1XMO
| Entry DOI | 10.2210/pdb1xmo/pdb |
| Related | 1FKA 1IBK 1IBL 1IBM 1XMQ 1XNQ 1XNR |
| Descriptor | 16S ribosomal RNA, 30S ribosomal protein S8, 30S ribosomal protein S9, ... (26 entities in total) |
| Functional Keywords | ribosome, decoding, trna modification |
| Biological source | Thermus thermophilus More |
| Total number of polymer chains | 23 |
| Total formula weight | 791605.01 |
| Authors | Murphy, F.V.,Ramakrishnan, V.,Malkiewicz, A.,Agris, P.F. (deposition date: 2004-10-04, release date: 2004-12-14, Last modification date: 2024-10-09) |
| Primary citation | Murphy, F.V.,Ramakrishnan, V.,Malkiewicz, A.,Agris, P.F. The role of modifications in codon discrimination by tRNA(Lys)(UUU). Nat.Struct.Mol.Biol., 11:1186-1191, 2004 Cited by PubMed Abstract: The natural modification of specific nucleosides in many tRNAs is essential during decoding of mRNA by the ribosome. For example, tRNA(Lys)(UUU) requires the modification N6-threonylcarbamoyladenosine at position 37 (t(6)A37), adjacent and 3' to the anticodon, to bind AAA in the A site of the ribosomal 30S subunit. Moreover, it can only bind both AAA and AAG lysine codons when doubly modified with t(6)A37 and either 5-methylaminomethyluridine or 2-thiouridine at the wobble position (mnm(5)U34 or s(2)U34). Here we report crystal structures of modified tRNA anticodon stem-loops bound to the 30S ribosomal subunit with lysine codons in the A site. These structures allow the rationalization of how modifications in the anticodon loop enable decoding of both lysine codons AAA and AAG. PubMed: 15558052DOI: 10.1038/nsmb861 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (3.25 Å) |
Structure validation
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