1FKA

STRUCTURE OF FUNCTIONALLY ACTIVATED SMALL RIBOSOMAL SUBUNIT AT 3.3 A RESOLUTION

Summary for 1FKA

• Entry DOI: 10.2210/pdb1fka/pdb
• Related: 1A32 1AN7 1C05 1EKC 1PKP 1QKF 1RIS 1RSS
• Descriptor: 16S RIBOSOMAL RNA, 30S RIBOSOMAL PROTEIN S10, 30S RIBOSOMAL PROTEIN S11, ... (21 entities in total)
• Functional Keywords: 30s ribosomal subunit, protein-rna complex, ribosome
• Biological source: Thermus thermophilus; More
• Total number of polymer chains: 20
• Total formula weight: 725011.66

Authors

Schluenzen, F.,Tocilj, A.,Zarivach, R.,Harms, J.,Gluehmann, M.,Janell, D.,Bashan, A.,Bartels, H.,Agmon, I.,Franceschi, F.,Yonath, A. (deposition date: 2000-08-09, release date: 2000-09-04, Last modification date: 2024-02-07)

Primary citation

Schluenzen, F.,Tocilj, A.,Zarivach, R.,Harms, J.,Gluehmann, M.,Janell, D.,Bashan, A.,Bartels, H.,Agmon, I.,Franceschi, F.,Yonath, A.
Structure of functionally activated small ribosomal subunit at 3.3 angstroms resolution.
Cell(Cambridge,Mass.), 102:615-623, 2000

PubMed Abstract: The small ribosomal subunit performs the decoding of genetic information during translation. The structure of that from Thermus thermophilus shows that the decoding center, which positions mRNA and three tRNAs, is constructed entirely of RNA. The entrance to the mRNA channel will encircle the message when a latch-like contact closes and contributes to processivity and fidelity. Extended RNA helical elements that run longitudinally through the body transmit structural changes, correlating events at the particle's far end with the cycle of mRNA translocation at the decoding region. 96% of the nucleotides were traced and the main fold of all proteins was determined. The latter are either peripheral or appear to serve as linkers. Some may assist the directionality of translocation.

PubMed: 11007480
DOI: 10.1016/S0092-8674(00)00084-2

Experimental method

X-RAY DIFFRACTION (3.3 Å)