1N32

Structure of the Thermus thermophilus 30S ribosomal subunit bound to codon and near-cognate transfer RNA anticodon stem-loop mismatched at the first codon position at the a site with paromomycin

Summary for 1N32

Related1J5E 1FJG 1IBL 1IBK 1IBM 1N33 1N34 1N36
Descriptor16S RIBOSOMAL RNA, ANTICODON STEM-LOOP OF LEU-2 TRANSFER RNA, A-SITE MESSENGER RNA FRAGMENT, ... (26 entities in total)
Functional Keywords30s ribosomal subunit, ribosome, a site, decoding, near-cognate, mismatch, wobble, gu, g:u, transfer rna, trna, anticodon, stem-loop, messenger rna, mrna, codon, antibiotic, paromomycin
Biological sourceThermus thermophilus
Total number of polymer chains23
Total molecular weight794340.21
Authors
Ogle, J.M.,Murphy IV, F.V.,Tarry, M.J.,Ramakrishnan, V. (deposition date: 2002-10-25, release date: 2002-11-29, Last modification date: 2011-07-13)
Primary citation
Ogle, J.M.,Murphy IV, F.V.,Tarry, M.J.,Ramakrishnan, V.
Selection of tRNA by the Ribosome Requires a Transition from an Open to a Closed Form
Cell(Cambridge,Mass.), 111:721-732, 2002
PubMed: 12464183 (PDB entries with the same primary citation)
DOI: 10.1016/S0092-8674(02)01086-3
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (3 Å)
NMR Information
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliersRNA backbone0.258327.4%9.6%7.5%0.66MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution

More Asymmetric unit images

Molmil generated image of 1n32
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Molmil generated image of 1n32
rotated about x axis by 90°
Molmil generated image of 1n32
rotated about y axis by 90°