1EG0

FITTING OF COMPONENTS WITH KNOWN STRUCTURE INTO AN 11.5 A CRYO-EM MAP OF THE E.COLI 70S RIBOSOME

Summary for 1EG0

• Entry DOI: 10.2210/pdb1eg0/pdb
• EMDB information: 1003
• Descriptor: FRAGMENT OF 16S RRNA HELIX 23, PROTEIN (S15 RIBOSOMAL PROTEIN), PROTEIN (S17 RIBOSOMAL PROTEIN), ... (15 entities in total)
• Functional Keywords: 70s ribosome, low resolution model, ribosome
• Biological source: Escherichia coli; More
• Total number of polymer chains: 15
• Total formula weight: 224438.31

Authors

Gabashvili, I.S.,Agrawal, R.K.,Spahn, C.M.T.,Grassucci, R.A.,Svergun, D.I.,Frank, J.,Penczek, P. (deposition date: 2000-02-11, release date: 2000-03-06, Last modification date: 2024-02-07)

Primary citation

Gabashvili, I.S.,Agrawal, R.K.,Spahn, C.M.,Grassucci, R.A.,Svergun, D.I.,Frank, J.,Penczek, P.
Solution structure of the E. coli 70S ribosome at 11.5 A resolution.
Cell(Cambridge,Mass.), 100:537-549, 2000

PubMed Abstract: Over 73,000 projections of the E. coli ribosome bound with formyl-methionyl initiator tRNAf(Met) were used to obtain an 11.5 A cryo-electron microscopy map of the complex. This map allows identification of RNA helices, peripheral proteins, and intersubunit bridges. Comparison of double-stranded RNA regions and positions of proteins identified in both cryo-EM and X-ray maps indicates good overall agreement but points to rearrangements of ribosomal components required for the subunit association. Fitting of known components of the 50S stalk base region into the map defines the architecture of the GTPase-associated center and reveals a major change in the orientation of the alpha-sarcin-ricin loop. Analysis of the bridging connections between the subunits provides insight into the dynamic signaling mechanism between the ribosomal subunits.

PubMed: 10721991
DOI: 10.1016/S0092-8674(00)80690-X

Experimental method

ELECTRON MICROSCOPY (11.5 Å)