1JGQ

The Path of Messenger RNA Through the Ribosome. THIS FILE, 1JGQ, CONTAINS THE 30S RIBOSOME SUBUNIT, THREE TRNA, AND MRNA MOLECULES. 50S RIBOSOME SUBUNIT IS IN THE FILE 1GIY

Summary for 1JGQ

Entry DOI	10.2210/pdb1jgq/pdb
Related	1GIX 1GIY 1JGO 1JGP 1JGQ
Descriptor	30S 16S ribosomal RNA, 30S RIBOSOMAL PROTEIN S7, 30S RIBOSOMAL PROTEIN S8, ... (24 entities in total)
Functional Keywords	ribosome assembly, protein synthesis, life, ribosome
Biological source	Thermus thermophilus More
Total number of polymer chains	25
Total formula weight	869012.52
Authors	Yusupova, G.Z.,Yusupov, M.M.,Cate, J.H.D.,Noller, H.F. (deposition date: 2001-06-26, release date: 2001-07-20, Last modification date: 2024-05-22)
Primary citation	Yusupova, G.Z.,Yusupov, M.M.,Cate, J.H.,Noller, H.F. The path of messenger RNA through the ribosome. Cell(Cambridge,Mass.), 106:233-241, 2001 Cited by PubMed Abstract: Using X-ray crystallography, we have directly observed the path of mRNA in the 70S ribosome in Fourier difference maps at 7 A resolution. About 30 nucleotides of the mRNA are wrapped in a groove that encircles the neck of the 30S subunit. The Shine-Dalgarno helix is bound in a large cleft between the head and the back of the platform. At the interface, only about eight nucleotides (-1 to +7), centered on the junction between the A and P codons, are exposed, and bond almost exclusively to 16S rRNA. The mRNA enters the ribosome around position +13 to +15, the location of downstream pseudoknots that stimulate -1 translational frame shifting. PubMed: 11511350 DOI: 10.1016/S0092-8674(01)00435-4 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (5 Å)

Structure validation