2CLT
Crystal structure of the active form (full-length) of human fibroblast collagenase.
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0004222 | molecular_function | metalloendopeptidase activity |
| A | 0006508 | biological_process | proteolysis |
| A | 0008237 | molecular_function | metallopeptidase activity |
| A | 0008270 | molecular_function | zinc ion binding |
| A | 0031012 | cellular_component | extracellular matrix |
| B | 0004222 | molecular_function | metalloendopeptidase activity |
| B | 0006508 | biological_process | proteolysis |
| B | 0008237 | molecular_function | metallopeptidase activity |
| B | 0008270 | molecular_function | zinc ion binding |
| B | 0031012 | cellular_component | extracellular matrix |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE CA A1101 |
| Chain | Residue |
| A | ASP105 |
| A | GLU180 |
| A | GLU182 |
| A | HOH2034 |
| B | GLN335 |
| site_id | AC2 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE CA A1102 |
| Chain | Residue |
| A | ASP266 |
| A | GLU310 |
| A | ASP359 |
| A | ASP408 |
| site_id | AC3 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE CA A1103 |
| Chain | Residue |
| A | ASP139 |
| A | GLY171 |
| A | GLY173 |
| A | ASP175 |
| A | HOH2044 |
| site_id | AC4 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE CA A1104 |
| Chain | Residue |
| A | ASP156 |
| A | GLY157 |
| A | GLY159 |
| A | ASN161 |
| A | ASP179 |
| A | GLU182 |
| site_id | AC5 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE ZN A1201 |
| Chain | Residue |
| A | HIS149 |
| A | ASP151 |
| A | HIS164 |
| A | HIS177 |
| site_id | AC6 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE ZN A1202 |
| Chain | Residue |
| A | HIS199 |
| A | HIS203 |
| A | HIS209 |
| A | HOH2106 |
| site_id | AC7 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE CA B1301 |
| Chain | Residue |
| A | HOH2084 |
| B | ASP105 |
| B | GLU180 |
| B | GLU182 |
| site_id | AC8 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE CA B1302 |
| Chain | Residue |
| B | ASP266 |
| B | GLU310 |
| B | ASP359 |
| B | ASP408 |
| site_id | AC9 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE CA B1303 |
| Chain | Residue |
| B | ALA138 |
| B | ASP139 |
| B | GLY171 |
| B | GLY173 |
| B | ASP175 |
| B | HOH2043 |
| site_id | BC1 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE CA B1304 |
| Chain | Residue |
| B | ASP156 |
| B | GLY157 |
| B | GLY159 |
| B | ASN161 |
| B | ASP179 |
| B | GLU182 |
| site_id | BC2 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE ZN B1401 |
| Chain | Residue |
| B | HIS149 |
| B | ASP151 |
| B | HIS164 |
| B | HIS177 |
| site_id | BC3 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE ZN B1402 |
| Chain | Residue |
| B | HIS199 |
| B | HIS203 |
| B | HIS209 |
| B | HOH2092 |
Functional Information from PROSITE/UniProt
| site_id | PS00024 |
| Number of Residues | 16 |
| Details | HEMOPEXIN Hemopexin domain signature. ISvfWpqlPnGLEAAY |
| Chain | Residue | Details |
| A | ILE298-TYR313 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 2 |
| Details | ACT_SITE: |
| Chain | Residue | Details |
| A | ALA200 | |
| B | ALA200 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:8031754, ECO:0007744|PDB:1CGE, ECO:0007744|PDB:1CGF, ECO:0007744|PDB:1SU3, ECO:0007744|PDB:2CLT, ECO:0007744|PDB:2J0T, ECO:0007744|PDB:3SHI, ECO:0007744|PDB:966C |
| Chain | Residue | Details |
| A | ASP105 | |
| B | ASP105 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 6 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:15611040, ECO:0000269|PubMed:7656013, ECO:0000269|PubMed:8031754, ECO:0007744|PDB:1CGE, ECO:0007744|PDB:1CGF, ECO:0007744|PDB:1SU3, ECO:0007744|PDB:2CLT, ECO:0007744|PDB:2J0T, ECO:0007744|PDB:2TCL, ECO:0007744|PDB:4AUO, ECO:0007744|PDB:966C |
| Chain | Residue | Details |
| A | ASP139 | |
| A | GLY171 | |
| A | ASP175 | |
| B | ASP139 | |
| B | GLY171 | |
| B | ASP175 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 12 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:15611040, ECO:0000269|PubMed:7656013, ECO:0000269|PubMed:8031754, ECO:0000269|PubMed:8090713, ECO:0000269|PubMed:8278810, ECO:0000269|PubMed:9484219, ECO:0007744|PDB:1AYK, ECO:0007744|PDB:1CGE, ECO:0007744|PDB:1CGF, ECO:0007744|PDB:1CGL, ECO:0007744|PDB:1HFC, ECO:0007744|PDB:1SU3, ECO:0007744|PDB:2AYK, ECO:0007744|PDB:2CLT, ECO:0007744|PDB:2J0T, ECO:0007744|PDB:2TCL, ECO:0007744|PDB:3AYK, ECO:0007744|PDB:3SHI, ECO:0007744|PDB:4AUO, ECO:0007744|PDB:4AYK, ECO:0007744|PDB:966C |
| Chain | Residue | Details |
| A | HIS149 | |
| B | HIS199 | |
| B | HIS203 | |
| B | HIS209 | |
| A | HIS164 | |
| A | HIS177 | |
| A | HIS199 | |
| A | HIS203 | |
| A | HIS209 | |
| B | HIS149 | |
| B | HIS164 | |
| B | HIS177 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:15611040, ECO:0000269|PubMed:7656013, ECO:0000269|PubMed:8031754, ECO:0000269|PubMed:8090713, ECO:0000269|PubMed:8278810, ECO:0000269|PubMed:9484219, ECO:0007744|PDB:1CGE, ECO:0007744|PDB:1CGF, ECO:0007744|PDB:1CGL, ECO:0007744|PDB:1HFC, ECO:0007744|PDB:1SU3, ECO:0007744|PDB:2AYK, ECO:0007744|PDB:2CLT, ECO:0007744|PDB:2J0T, ECO:0007744|PDB:2TCL, ECO:0007744|PDB:3SHI, ECO:0007744|PDB:4AUO, ECO:0007744|PDB:966C |
| Chain | Residue | Details |
| A | ASP151 | |
| B | ASP151 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:15611040, ECO:0000269|PubMed:7656013, ECO:0000269|PubMed:8031754, ECO:0000269|PubMed:8090713, ECO:0000269|PubMed:8278810, ECO:0007744|PDB:1CGE, ECO:0007744|PDB:1CGF, ECO:0007744|PDB:1CGL, ECO:0007744|PDB:1HFC, ECO:0007744|PDB:1SU3, ECO:0007744|PDB:2CLT, ECO:0007744|PDB:2J0T, ECO:0007744|PDB:2TCL, ECO:0007744|PDB:3SHI, ECO:0007744|PDB:4AUO, ECO:0007744|PDB:966C |
| Chain | Residue | Details |
| A | ASP156 | |
| B | ASP156 |
| site_id | SWS_FT_FI7 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:15611040, ECO:0000269|PubMed:7656013, ECO:0000269|PubMed:8031754, ECO:0000269|PubMed:8090713, ECO:0000269|PubMed:8278810, ECO:0000269|PubMed:9484219, ECO:0007744|PDB:1AYK, ECO:0007744|PDB:1CGE, ECO:0007744|PDB:1CGF, ECO:0007744|PDB:1CGL, ECO:0007744|PDB:1HFC, ECO:0007744|PDB:1SU3, ECO:0007744|PDB:2AYK, ECO:0007744|PDB:2CLT, ECO:0007744|PDB:2J0T, ECO:0007744|PDB:2TCL, ECO:0007744|PDB:3SHI, ECO:0007744|PDB:4AUO, ECO:0007744|PDB:4AYK, ECO:0007744|PDB:966C |
| Chain | Residue | Details |
| A | GLY157 | |
| B | GLY157 |
| site_id | SWS_FT_FI8 |
| Number of Residues | 4 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:15611040, ECO:0000269|PubMed:7656013, ECO:0000269|PubMed:8031754, ECO:0000269|PubMed:8090713, ECO:0000269|PubMed:8278810, ECO:0000269|PubMed:9484219, ECO:0007744|PDB:1AYK, ECO:0007744|PDB:1CGE, ECO:0007744|PDB:1CGF, ECO:0007744|PDB:1CGL, ECO:0007744|PDB:1HFC, ECO:0007744|PDB:1SU3, ECO:0007744|PDB:2AYK, ECO:0007744|PDB:2CLT, ECO:0007744|PDB:2J0T, ECO:0007744|PDB:2TCL, ECO:0007744|PDB:3SHI, ECO:0007744|PDB:4AUO, ECO:0007744|PDB:966C |
| Chain | Residue | Details |
| A | GLY159 | |
| A | ASN161 | |
| B | GLY159 | |
| B | ASN161 |
| site_id | SWS_FT_FI9 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:7656013, ECO:0000269|PubMed:8031754, ECO:0007744|PDB:1CGE, ECO:0007744|PDB:1CGF, ECO:0007744|PDB:2CLT, ECO:0007744|PDB:2J0T, ECO:0007744|PDB:2TCL, ECO:0007744|PDB:4AUO, ECO:0007744|PDB:966C |
| Chain | Residue | Details |
| A | GLY173 | |
| B | GLY173 |
| site_id | SWS_FT_FI10 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:15611040, ECO:0000269|PubMed:7656013, ECO:0000269|PubMed:8031754, ECO:0000269|PubMed:8090713, ECO:0000269|PubMed:8278810, ECO:0007744|PDB:1CGE, ECO:0007744|PDB:1CGF, ECO:0007744|PDB:1CGL, ECO:0007744|PDB:1HFC, ECO:0007744|PDB:1SU3, ECO:0007744|PDB:2CLT, ECO:0007744|PDB:2J0T, ECO:0007744|PDB:2TCL, ECO:0007744|PDB:3AYK, ECO:0007744|PDB:3SHI, ECO:0007744|PDB:4AUO, ECO:0007744|PDB:4AYK, ECO:0007744|PDB:966C |
| Chain | Residue | Details |
| A | ASP179 | |
| B | ASP179 |
| site_id | SWS_FT_FI11 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:15611040, ECO:0000269|PubMed:8031754, ECO:0007744|PDB:1CGE, ECO:0007744|PDB:1CGF, ECO:0007744|PDB:1SU3, ECO:0007744|PDB:2CLT, ECO:0007744|PDB:2J0T, ECO:0007744|PDB:3SHI, ECO:0007744|PDB:4AUO, ECO:0007744|PDB:966C |
| Chain | Residue | Details |
| A | GLU180 | |
| B | GLU180 |
| site_id | SWS_FT_FI12 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:15611040, ECO:0000269|PubMed:7656013, ECO:0000269|PubMed:8031754, ECO:0000269|PubMed:8090713, ECO:0000269|PubMed:8278810, ECO:0000269|PubMed:9484219, ECO:0007744|PDB:1AYK, ECO:0007744|PDB:1CGE, ECO:0007744|PDB:1CGF, ECO:0007744|PDB:1CGL, ECO:0007744|PDB:1HFC, ECO:0007744|PDB:1SU3, ECO:0007744|PDB:2AYK, ECO:0007744|PDB:2CLT, ECO:0007744|PDB:2J0T, ECO:0007744|PDB:2TCL, ECO:0007744|PDB:3AYK, ECO:0007744|PDB:3SHI, ECO:0007744|PDB:4AUO, ECO:0007744|PDB:966C |
| Chain | Residue | Details |
| A | GLU182 | |
| B | GLU182 |
| site_id | SWS_FT_FI13 |
| Number of Residues | 8 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:15611040, ECO:0007744|PDB:1SU3, ECO:0007744|PDB:2CLT, ECO:0007744|PDB:4AUO |
| Chain | Residue | Details |
| A | ASP266 | |
| A | GLU310 | |
| A | ASP359 | |
| A | ASP408 | |
| B | ASP266 | |
| B | GLU310 | |
| B | ASP359 | |
| B | ASP408 |
| site_id | SWS_FT_FI14 |
| Number of Residues | 2 |
| Details | SITE: Not glycosylated => ECO:0000269|PubMed:10092871 |
| Chain | Residue | Details |
| A | ASN124 | |
| B | ASN124 |
| site_id | SWS_FT_FI15 |
| Number of Residues | 2 |
| Details | SITE: Cleavage; by autolysis => ECO:0000269|PubMed:2557822 |
| Chain | Residue | Details |
| A | PRO250 | |
| B | PRO250 |
| site_id | SWS_FT_FI16 |
| Number of Residues | 2 |
| Details | MOD_RES: Phosphothreonine => ECO:0000269|PubMed:25171405 |
| Chain | Residue | Details |
| A | THR255 | |
| B | THR255 |
| site_id | SWS_FT_FI17 |
| Number of Residues | 2 |
| Details | MOD_RES: Phosphotyrosine; by PKDCC => ECO:0000269|PubMed:25171405 |
| Chain | Residue | Details |
| A | TYR341 | |
| B | TYR341 |
| site_id | SWS_FT_FI18 |
| Number of Residues | 2 |
| Details | CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269|PubMed:10092871 |
| Chain | Residue | Details |
| A | ASN101 | |
| B | ASN101 |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1hfs |
| Chain | Residue | Details |
| A | ALA200 | |
| A | MET217 |
| site_id | CSA2 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1hfs |
| Chain | Residue | Details |
| B | ALA200 | |
| B | MET217 |
| site_id | CSA3 |
| Number of Residues | 1 |
| Details | Annotated By Reference To The Literature 1hfs |
| Chain | Residue | Details |
| A | ALA200 |
| site_id | CSA4 |
| Number of Residues | 1 |
| Details | Annotated By Reference To The Literature 1hfs |
| Chain | Residue | Details |
| B | ALA200 |
