Mass: 23206.619 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) TOXOPLASMA GONDII (eukaryote) / Gene: 541.M00136 / Plasmid: P15-MHL / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): DH5A / References: UniProt: D0VWS5
#2: Protein/peptide
CYCLOSPORINA / CICLOSPORIN / CICLOSPORINE
Type: Cyclic peptide / Class: Immunosuppressant / Mass: 1220.625 Da / Num. of mol.: 4 / Source method: obtained synthetically Details: CYCLOSPORIN IS A CYCLIC UNDECAPEPTIDE. CYCLIZATION IS ACHIEVED BY LINKING THE N- AND THE C- TERMINI. Source: (synth.) TOLYPOCLADIUM INFLATUM (fungus) / References: NOR: NOR00033, Cyclosporin A
Mass: 18.015 Da / Num. of mol.: 484 / Source method: isolated from a natural source / Formula: H2O
Compound details
CYCLOSPORIN IS A CYCLIC UNDECAPEPTIDE. HERE, CYCLOSPORIN A IS REPRESENTED BY THE SEQUENCE (SEQRES)
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.98 Å3/Da / Density % sol: 58.76 %
Crystal grow
pH: 7.3 Details: 28% PEG 3350, 0.2 M LISO4, 0.1 M HEPES PH 7.3, 2 MM TCEP, 2 MM CYCLOSPORIN A, 20% ETHYLENE GLYCOL, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 293K