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Yorodumi- PDB-2rmc: Crystal structure of murine cyclophilin C complexed with immunosu... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2rmc | |||||||||
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Title | Crystal structure of murine cyclophilin C complexed with immunosuppressive drug cyclosporin A | |||||||||
Components |
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Keywords | ISOMERASE/IMMUNOSUPPRESSANT / ISOMERASE-IMMUNOSUPPRESSANT COMPLEX / CYCLOPHILIN-CYCLOSPORIN COMPLEX / CYCLOSPORIN A / IMMUNOSUPPRESSANT / CYCLOPHILIN | |||||||||
Function / homology | Function and homology information cyclosporin A binding / protein peptidyl-prolyl isomerization / peptidylprolyl isomerase / peptidyl-prolyl cis-trans isomerase activity / protein folding / intracellular membrane-bounded organelle / cytoplasm Similarity search - Function | |||||||||
Biological species | MUS MUSCULUS (house mouse) TOLYPOCLADIUM INFLATUM (fungus) | |||||||||
Method | X-RAY DIFFRACTION / Resolution: 1.64 Å | |||||||||
Authors | Ke, H. / Zhao, Y. / Luo, F. / Weissman, I. / Friedman, J. | |||||||||
Citation | Journal: Proc.Natl.Acad.Sci.USA / Year: 1993 Title: Crystal Structure of Murine Cyclophilin C Complexed with Immunosuppressive Drug Cyclosporin A Authors: Ke, H. / Zhao, Y. / Luo, F. / Weissman, I. / Friedman, J. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2rmc.cif.gz | 205.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2rmc.ent.gz | 168.8 KB | Display | PDB format |
PDBx/mmJSON format | 2rmc.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/rm/2rmc ftp://data.pdbj.org/pub/pdb/validation_reports/rm/2rmc | HTTPS FTP |
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-Related structure data
Related structure data | |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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3 |
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4 |
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Unit cell |
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Atom site foot note | 1: RESIDUE ALA 8 IN CHAINS B, D, F, AND H IS A D-ALANINE. |
-Components
#1: Protein | Mass: 19941.682 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) MUS MUSCULUS (house mouse) / References: UniProt: P30412, peptidylprolyl isomerase #2: Protein/peptide | Type: Cyclic peptide / Class: ImmunosuppressantImmunosuppressive drug / Mass: 1220.625 Da / Num. of mol.: 4 / Source method: obtained synthetically Details: CYCLOSPORIN IS A CYCLIC UNDECAPEPTIDE. CYCLIZATION IS ACHIEVED BY LINKING THE N- AND THE C- TERMINI. Source: (synth.) TOLYPOCLADIUM INFLATUM (fungus) / References: NOR: NOR00033, Cyclosporin A #3: Water | ChemComp-HOH / | Compound details | CYCLOSPORI | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.58 Å3/Da / Density % sol: 52.31 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | *PLUS Temperature: 4 ℃ / pH: 6 / Method: microdialysis | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
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Radiation wavelength | Relative weight: 1 |
Reflection | *PLUS Highest resolution: 1.64 Å / Num. obs: 73850 / Num. measured all: 139812 / Rmerge(I) obs: 0.04 |
-Processing
Software |
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Refinement | Resolution: 1.64→6 Å Details: THE SPACE GROUP IS P21 WITH THE ORIGIN AT 1/4 OF X SO THAT THE SYMMETRY IS (X,Y,Z) AND (1/2-X, 1/2+Y,-X).
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Refinement step | Cycle: LAST / Resolution: 1.64→6 Å
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Refine LS restraints |
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Software | *PLUS Name: X-PLOR / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Rfactor obs: 0.197 / Rfactor Rwork: 0.197 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS Type: x_angle_d / Dev ideal: 2.68 |