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- PDB-2x5q: Crystal Structure of Hypothetical protein sso1986 from Sulfolobus... -
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Open data
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Basic information
Entry | Database: PDB / ID: 2x5q | ||||||
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Title | Crystal Structure of Hypothetical protein sso1986 from Sulfolobus solfataricus P2 | ||||||
![]() | SSO1986 | ||||||
![]() | UNKNOWN FUNCTION | ||||||
Function / homology | CRISPR system CMR subunit Cmr7 1 / defense response to virus / cytoplasm / CRISPR system CMR subunit Cmr7 1![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Oke, M. / Carter, L. / Johnson, K.A. / Kerou, M. / Liu, H. / Mcmahon, S. / Naismith, J.H. / White, M.F. | ||||||
![]() | ![]() Title: The Scottish Structural Proteomics Facility: Targets, Methods and Outputs. Authors: Oke, M. / Carter, L.G. / Johnson, K.A. / Liu, H. / Mcmahon, S.A. / Yan, X. / Kerou, M. / Weikart, N.D. / Kadi, N. / Sheikh, M.A. / Schmelz, S. / Dorward, M. / Zawadzki, M. / Cozens, C. / ...Authors: Oke, M. / Carter, L.G. / Johnson, K.A. / Liu, H. / Mcmahon, S.A. / Yan, X. / Kerou, M. / Weikart, N.D. / Kadi, N. / Sheikh, M.A. / Schmelz, S. / Dorward, M. / Zawadzki, M. / Cozens, C. / Falconer, H. / Powers, H. / Overton, I.M. / Van Niekerk, C.A.J. / Peng, X. / Patel, P. / Garrett, R.A. / Prangishvili, D. / Botting, C.H. / Coote, P.J. / Dryden, D.T.F. / Barton, G.J. / Schwarz-Linek, U. / Challis, G.L. / Taylor, G.L. / White, M.F. / Naismith, J.H. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 162.5 KB | Display | ![]() |
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PDB format | ![]() | 130.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 434 KB | Display | ![]() |
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Full document | ![]() | 437.4 KB | Display | |
Data in XML | ![]() | 16.8 KB | Display | |
Data in CIF | ![]() | 23.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 2ivyC ![]() 2jg5C ![]() 2jg6C ![]() 2vw8C ![]() 2vxzC ![]() 2wj9C ![]() 2x0oC ![]() 2x3dC ![]() 2x3eC ![]() 2x3fC ![]() 2x3gC ![]() 2x3lC ![]() 2x3mC ![]() 2x3nC ![]() 2x3oC ![]() 2x48C ![]() 2x4gC ![]() 2x4hC ![]() 2x4iC ![]() 2x4jC ![]() 2x4kC ![]() 2x4lC ![]() 2x5cC ![]() 2x5dC ![]() 2x5fC ![]() 2x5gC ![]() 2x5hC ![]() 2x5pC ![]() 2x5rC ![]() 2x5tC ![]() 2x7bC ![]() 2x7iC ![]() 2xu2C C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components on special symmetry positions |
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Noncrystallographic symmetry (NCS) | NCS domain:
NCS domain segments:
NCS ensembles :
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Components
#1: Protein | Mass: 22235.197 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.97 Å3/Da / Density % sol: 37.41 % / Description: NONE |
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Crystal grow | pH: 4 Details: 19.2% PEG 6000, 0.1M NA-CITRATE, PH4.0, 0.93M LICL2, 9% ETHYLENE GLYCOL FREEZING SOLUTION: 21.0% PEG 6000, 0.1M NA-CITRATE, PH4.0, 0.5M LICL2, 25% ETHYLENE GLYCOL.DERIVATIVE CRYSTALS FOR SAD ...Details: 19.2% PEG 6000, 0.1M NA-CITRATE, PH4.0, 0.93M LICL2, 9% ETHYLENE GLYCOL FREEZING SOLUTION: 21.0% PEG 6000, 0.1M NA-CITRATE, PH4.0, 0.5M LICL2, 25% ETHYLENE GLYCOL.DERIVATIVE CRYSTALS FOR SAD PHASING WERE OBTAINED BY SOAKING THE CRYSTALS IN 200 MM TRIMETHYLLEAD CHLORIDE, 500 MM POTASSIUM OSMATE AND 200 MM DIPOTASSIUM HEXACHLORO OSMATE |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC CCD / Detector: CCD / Date: Sep 2, 2007 / Details: MIRRORS |
Radiation | Monochromator: SI(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.976 Å / Relative weight: 1 |
Reflection | Resolution: 2.05→29.54 Å / Num. obs: 20562 / % possible obs: 99 % / Observed criterion σ(I): 0 / Redundancy: 4.1 % / Biso Wilson estimate: 0 Å2 / Rmerge(I) obs: 0.06 / Net I/σ(I): 4.5 |
Reflection shell | Resolution: 2.05→2.1 Å / Redundancy: 3.8 % / Rmerge(I) obs: 0.35 / Mean I/σ(I) obs: 4.5 / % possible all: 97.7 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: NONE Resolution: 2.05→29.54 Å / Cor.coef. Fo:Fc: 0.951 / Cor.coef. Fo:Fc free: 0.933 / SU B: 9.469 / SU ML: 0.131 / Cross valid method: THROUGHOUT / ESU R: 0.244 / ESU R Free: 0.185 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. ATOM RECORD CONTAINS SUM OF TLS AND RESIDUAL B FACTORS. ANISOU RECORD CONTAINS SUM OF TLS AND RESIDUAL U FACTORS.
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: BABINET MODEL WITH MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 28.249 Å2
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Refinement step | Cycle: LAST / Resolution: 2.05→29.54 Å
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Refine LS restraints |
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