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Open data
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Basic information
Entry | Database: PDB / ID: 2es0 | ||||||
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Title | Structure of the regulator of G-protein signaling domain of RGS6 | ||||||
![]() | regulator of G-protein signalling 6 | ||||||
![]() | SIGNALING PROTEIN / HUMAN RGS6 / REGULATOR OF G-PROTEIN SIGNALING 6 / GTPASE-ACTIVATING PROTEINS (GAP) / DOMAIN SWAP / STRUCTURAL GENOMICS / STRUCTURAL GENOMICS CONSORTIUM / SGC | ||||||
Function / homology | ![]() regulation of G protein-coupled receptor signaling pathway / extrinsic component of membrane / negative regulation of signal transduction / GTPase activator activity / positive regulation of GTPase activity / Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding / G alpha (i) signalling events / intracellular signal transduction / G protein-coupled receptor signaling pathway / GTPase activity ...regulation of G protein-coupled receptor signaling pathway / extrinsic component of membrane / negative regulation of signal transduction / GTPase activator activity / positive regulation of GTPase activity / Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding / G alpha (i) signalling events / intracellular signal transduction / G protein-coupled receptor signaling pathway / GTPase activity / nucleus / plasma membrane / cytosol Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Schoch, G.A. / Phillips, C. / Turnbull, A. / Niesen, F. / Johansson, C. / Elkins, J.M. / Longman, E. / Gilealdi, C. / Sobott, F. / Ball, L. ...Schoch, G.A. / Phillips, C. / Turnbull, A. / Niesen, F. / Johansson, C. / Elkins, J.M. / Longman, E. / Gilealdi, C. / Sobott, F. / Ball, L. / Sundstrom, M. / Edwards, A. / Arrowsmith, C. / von Delft, F. / Doyle, D.A. / Structural Genomics Consortium (SGC) | ||||||
![]() | ![]() Title: Structural diversity in the RGS domain and its interaction with heterotrimeric G protein alpha-subunits. Authors: Soundararajan, M. / Willard, F.S. / Kimple, A.J. / Turnbull, A.P. / Ball, L.J. / Schoch, G.A. / Gileadi, C. / Fedorov, O.Y. / Dowler, E.F. / Higman, V.A. / Hutsell, S.Q. / Sundstrom, M. / ...Authors: Soundararajan, M. / Willard, F.S. / Kimple, A.J. / Turnbull, A.P. / Ball, L.J. / Schoch, G.A. / Gileadi, C. / Fedorov, O.Y. / Dowler, E.F. / Higman, V.A. / Hutsell, S.Q. / Sundstrom, M. / Doyle, D.A. / Siderovski, D.P. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 43.7 KB | Display | ![]() |
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PDB format | ![]() | 29.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 418.1 KB | Display | ![]() |
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Full document | ![]() | 418.2 KB | Display | |
Data in XML | ![]() | 8.3 KB | Display | |
Data in CIF | ![]() | 11.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1zv4C ![]() 2a72SC ![]() 2af0C ![]() 2bt2C ![]() 2bv1C ![]() 2gtpC ![]() 2i59C ![]() 2ihbC ![]() 2ihdC ![]() 2ik8C ![]() 2jm5C ![]() 2jnuC ![]() 2odeC ![]() 2owiC S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components on special symmetry positions |
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Details | Dimer, with domain swap Form a biological unit with symmetry related molecule (X, Y, -Z) |
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Components
#1: Protein | Mass: 17236.523 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.64 Å3/Da / Density % sol: 66.22 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop Details: PEG 10K; (NH4)(ac); BIS-TRIS , pH pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARMOSAIC 225 mm CCD / Detector: CCD / Date: Oct 1, 2005 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.987 Å / Relative weight: 1 |
Reflection | Resolution: 2.1→72.93 Å / Num. all: 15210 / Num. obs: 15210 / % possible obs: 99.9 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 7.1 % / Biso Wilson estimate: 43.143 Å2 / Rmerge(I) obs: 0.083 / Rsym value: 0.089 / Net I/σ(I): 15.4 |
Reflection shell | Resolution: 2.1→2.21 Å / Redundancy: 5.6 % / Rmerge(I) obs: 0.01088 / Mean I/σ(I) obs: 1.7 / Num. unique all: 2183 / Rsym value: 0.01204 / % possible all: 100 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 2A72.pdb Resolution: 2.1→67.12 Å / Cor.coef. Fo:Fc: 0.961 / Cor.coef. Fo:Fc free: 0.946 / SU B: 8.654 / SU ML: 0.122 / TLS residual ADP flag: LIKELY RESIDUAL / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R: 0.148 / ESU R Free: 0.142 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: BABINET MODEL WITH MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 43.143 Å2
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Refinement step | Cycle: LAST / Resolution: 2.1→67.12 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.1→2.155 Å / Total num. of bins used: 20
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Refinement TLS params. | Method: refined / Origin x: 13.3 Å / Origin y: 35.5591 Å / Origin z: 2.5897 Å
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