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- PDB-2ihd: Crystal structure of Human Regulator of G-protein signaling 8, RGS8 -
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Open data
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Basic information
Entry | Database: PDB / ID: 2ihd | ||||||
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Title | Crystal structure of Human Regulator of G-protein signaling 8, RGS8 | ||||||
![]() | Regulator of G-protein signaling 8 | ||||||
![]() | SIGNALING PROTEIN / RGS8 / RGS / Structural Genomics / Structural Genomics Consortium / SGC | ||||||
Function / homology | ![]() regulation of dopamine receptor signaling pathway / neuronal cell body membrane / negative regulation of signal transduction / GTPase activator activity / positive regulation of GTPase activity / G protein-coupled acetylcholine receptor signaling pathway / cytoplasmic side of plasma membrane / G alpha (i) signalling events / perikaryon / G protein-coupled receptor signaling pathway ...regulation of dopamine receptor signaling pathway / neuronal cell body membrane / negative regulation of signal transduction / GTPase activator activity / positive regulation of GTPase activity / G protein-coupled acetylcholine receptor signaling pathway / cytoplasmic side of plasma membrane / G alpha (i) signalling events / perikaryon / G protein-coupled receptor signaling pathway / GTPase activity / synapse / dendrite / nucleus / plasma membrane Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Turnbull, A.P. / Papagrigoriou, E. / Ugochukwu, E. / Salah, E. / Gileadi, C. / Burgess, N. / Bhatia, C. / Gileadi, O. / Bray, J. / Elkins, J. ...Turnbull, A.P. / Papagrigoriou, E. / Ugochukwu, E. / Salah, E. / Gileadi, C. / Burgess, N. / Bhatia, C. / Gileadi, O. / Bray, J. / Elkins, J. / von Delft, F. / Weigelt, J. / Edwards, A. / Arrowsmith, C. / Sundstrom, M. / Doyle, D.A. / Structural Genomics Consortium (SGC) | ||||||
![]() | ![]() Title: Structural diversity in the RGS domain and its interaction with heterotrimeric G protein alpha-subunits. Authors: Soundararajan, M. / Willard, F.S. / Kimple, A.J. / Turnbull, A.P. / Ball, L.J. / Schoch, G.A. / Gileadi, C. / Fedorov, O.Y. / Dowler, E.F. / Higman, V.A. / Hutsell, S.Q. / Sundstrom, M. / ...Authors: Soundararajan, M. / Willard, F.S. / Kimple, A.J. / Turnbull, A.P. / Ball, L.J. / Schoch, G.A. / Gileadi, C. / Fedorov, O.Y. / Dowler, E.F. / Higman, V.A. / Hutsell, S.Q. / Sundstrom, M. / Doyle, D.A. / Siderovski, D.P. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 46 KB | Display | ![]() |
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PDB format | ![]() | 31.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 423.8 KB | Display | ![]() |
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Full document | ![]() | 424 KB | Display | |
Data in XML | ![]() | 8.9 KB | Display | |
Data in CIF | ![]() | 12.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1zv4C ![]() 2a72C ![]() 2af0C ![]() 2bt2SC ![]() 2bv1C ![]() 2es0C ![]() 2gtpC ![]() 2i59C ![]() 2ihbC ![]() 2ik8C ![]() 2jm5C ![]() 2jnuC ![]() 2odeC ![]() 2owiC S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 18232.605 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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#2: Chemical | ChemComp-CL / |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.28 Å3/Da / Density % sol: 46.01 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop Details: 0.20M NaI; 20.0% PEG 3350; 10.0% Ethylene Glycol, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARMOSAIC 225 mm CCD / Detector: CCD / Date: Aug 16, 2006 |
Radiation | Monochromator: Si (111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.978 Å / Relative weight: 1 |
Reflection | Resolution: 1.7→50 Å / Num. all: 17206 / Num. obs: 17199 / % possible obs: 95.7 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 |
Reflection shell | Resolution: 1.7→1.76 Å / % possible all: 99.7 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: Swissmodel based on pdb entry 2BT2 Resolution: 1.7→27.5 Å / Cor.coef. Fo:Fc: 0.962 / Cor.coef. Fo:Fc free: 0.95 / SU B: 3.803 / SU ML: 0.067 / TLS residual ADP flag: LIKELY RESIDUAL / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R: 0.101 / ESU R Free: 0.102 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: MASK | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 12.144 Å2
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Refinement step | Cycle: LAST / Resolution: 1.7→27.5 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.7→1.746 Å / Total num. of bins used: 20
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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