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- PDB-2owi: Solution structure of the RGS domain from human RGS18 -

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Basic information

Entry
Database: PDB / ID: 2owi
TitleSolution structure of the RGS domain from human RGS18
ComponentsRegulator of G-protein signaling 18
KeywordsSIGNALING PROTEIN / STRUCTURAL GENOMICS / STRUCTURAL GENOMICS CONSORTIUM / SGC
Function / homology
Function and homology information


regulation of G protein-coupled receptor signaling pathway / negative regulation of signal transduction / GTPase activator activity / G alpha (i) signalling events / G alpha (q) signalling events / G protein-coupled receptor signaling pathway / GTPase activity / plasma membrane / cytoplasm
Similarity search - Function
Regulator of G-protein signalling 18 / Regulator of G-protein Signalling 4; domain 1 - #10 / Regulator of G-protein Signalling 4; domain 1 / RGS, subdomain 1/3 / Regulator of G-protein Signalling 4, domain 2 / Regulator of G-protein Signalling 4; domain 2 / Regulator of G protein signaling domain / RGS, subdomain 2 / RGS domain / RGS domain profile. ...Regulator of G-protein signalling 18 / Regulator of G-protein Signalling 4; domain 1 - #10 / Regulator of G-protein Signalling 4; domain 1 / RGS, subdomain 1/3 / Regulator of G-protein Signalling 4, domain 2 / Regulator of G-protein Signalling 4; domain 2 / Regulator of G protein signaling domain / RGS, subdomain 2 / RGS domain / RGS domain profile. / Regulator of G protein signalling domain / RGS domain superfamily / Orthogonal Bundle / Mainly Alpha
Similarity search - Domain/homology
Regulator of G-protein signaling 18
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodSOLUTION NMR / SIMULATED ANNEALING, MOLECULAR DYNAMICS
AuthorsHigman, V.A. / Leidert, M. / Bray, J. / Elkins, J. / Soundararajan, M. / Doyle, D.A. / Gileadi, C. / Phillips, C. / Schoch, G. / Yang, X. ...Higman, V.A. / Leidert, M. / Bray, J. / Elkins, J. / Soundararajan, M. / Doyle, D.A. / Gileadi, C. / Phillips, C. / Schoch, G. / Yang, X. / Brockmann, C. / Schmieder, P. / Diehl, A. / Sundstrom, M. / Arrowsmith, C. / Weigelt, J. / Edwards, A. / Oschkinat, H. / Ball, L.J. / Structural Genomics Consortium (SGC)
Citation
Journal: Proc.Natl.Acad.Sci.Usa / Year: 2008
Title: Structural diversity in the RGS domain and its interaction with heterotrimeric G protein alpha-subunits.
Authors: Soundararajan, M. / Willard, F.S. / Kimple, A.J. / Turnbull, A.P. / Ball, L.J. / Schoch, G.A. / Gileadi, C. / Fedorov, O.Y. / Dowler, E.F. / Higman, V.A. / Hutsell, S.Q. / Sundstrom, M. / ...Authors: Soundararajan, M. / Willard, F.S. / Kimple, A.J. / Turnbull, A.P. / Ball, L.J. / Schoch, G.A. / Gileadi, C. / Fedorov, O.Y. / Dowler, E.F. / Higman, V.A. / Hutsell, S.Q. / Sundstrom, M. / Doyle, D.A. / Siderovski, D.P.
History
DepositionFeb 16, 2007Deposition site: RCSB / Processing site: RCSB
Revision 1.0Feb 27, 2007Provider: repository / Type: Initial release
Revision 1.1May 1, 2008Group: Version format compliance
Revision 1.2Jul 13, 2011Group: Version format compliance
SupersessionAug 15, 2012ID: 2H33
Revision 1.3Aug 15, 2012Group: Other

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: Regulator of G-protein signaling 18


Theoretical massNumber of molelcules
Total (without water)17,7861
Polymers17,7861
Non-polymers00
Water0
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
NMR ensembles
DataCriteria
Number of conformers (submitted / calculated)20 / 200structures with the lowest energy
RepresentativeModel #1lowest energy

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Components

#1: Protein Regulator of G-protein signaling 18 / RGS18


Mass: 17786.023 Da / Num. of mol.: 1 / Fragment: RGS DOMAIN, RESIDUES 75-223
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human)
Description: BL21 (DE3) STRAIN ENRICHED WITH GENES THAT ENCODE RARE TRNAS
Gene: RGS18, RGS13 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21 (DE3) - ROSETTA / References: UniProt: Q9NS28

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Experimental details

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Experiment

ExperimentMethod: SOLUTION NMR
NMR experiment
Conditions-IDExperiment-IDSolution-IDType
1122D 1H-15N HSQC
1223D HNCO
1323D CBCA(CO)NNH
1423D CBCACONNH
1523D HN(CA)CO
1623D H(CCO)NNH
1723D HBHA(CO)NNH
1823D (H)CCH-TOCSY
1923D (H)CCH-COSY
11013D 1H-15N NOESY HSQC
11123D 1H-13C NOESY HSQC
11233D 1H-13C HMQC NOESY
11332D 1H-13C HSQC
11432D TOCSY

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Sample preparation

Details
Solution-IDContentsSolvent system
11 MM [U-95% 15N] RGS18, 20 MM SODIUM PHOSPHATE, 50 MM SODIUM CHLORIDE, 10 % D2O, 1 MM [U- 99% 2H] DTT, 0.02 % SODIUM AZIDE90% H2O/10% D2O
21 MM [U-95% 13C, U-95% 15N] RGS18, 20 MM POTASSIUM PHOSPHATE, 50 MM SODIUM CHLORIDE, 10 % D2O, 1 MM [U- 99% 2H] DTT, 0.02 % SODIUM AZIDE90% H2O/10% D2O
31 MM [U-95% 13C, U-95% 15N] RGS18, 20 MM POTASSIUM PHOSPHATE, 50 MM SODIUM CHLORIDE, 100 % D2O, 1 MM [U- 99% 2H] DTT, 0.02 % SODIUM AZIDE100% D2O
Sample conditionspH: 6.0 / Pressure: AMBIENT / Temperature: 297 K

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NMR measurement

RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthRelative weight: 1
NMR spectrometer
TypeManufacturerModelField strength (MHz)Spectrometer-ID
Bruker DRXBrukerDRX6001
Bruker DMXBrukerDMX7502

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Processing

NMR software
NameVersionClassification
CNS1.1refinement
XWINNMR 2.6 AND3.1structure solution
CCPNMR_ ANALYSIS 1.0.9-101.0.9-10structure solution
CYANA2structure solution
X-PLOR_NIH2.14structure solution
RefinementMethod: SIMULATED ANNEALING, MOLECULAR DYNAMICS / Software ordinal: 1
Details: NOE ASSIGNMENT WITH CYANA, REFINEMENT BY SIMULATED ANNEALING IN XPLOR-NIH USING NOE RESTRAINTS AND H-BOND RESTRAINTS FROM H/D EXCHANGE DATA. WATER REFINEMENT IN CNS. THE C-TERMINAL TAIL HAS ...Details: NOE ASSIGNMENT WITH CYANA, REFINEMENT BY SIMULATED ANNEALING IN XPLOR-NIH USING NOE RESTRAINTS AND H-BOND RESTRAINTS FROM H/D EXCHANGE DATA. WATER REFINEMENT IN CNS. THE C-TERMINAL TAIL HAS BEEN TRUNCATED PAST RESIDUE 134 BECAUSE OF FLEXIBILITY AND LACK OF STRUCTURE IN THIS REGION. THIS IS INDICATED (A) BY A LACK OF NOE RESTRAINTS PAST RESIDUE 132 AND (B) BY 15N T1, T2 AND HETERONCULEAR NOE EXPERIMENTS RESIDUE 131 ONWARDS.
NMR representativeSelection criteria: lowest energy
NMR ensembleConformer selection criteria: structures with the lowest energy
Conformers calculated total number: 200 / Conformers submitted total number: 20

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