+Open data
-Basic information
Entry | Database: PDB / ID: 7cng | ||||||
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Title | Structure of CDK5R1 bound FEM1B | ||||||
Components | Protein fem-1 homolog B,Peptide from Cyclin-dependent kinase 5 activator 1 | ||||||
Keywords | PEPTIDE BINDING PROTEIN / ubiquitination / E3 ligase / degron | ||||||
Function / homology | Function and homology information superior olivary nucleus maturation / protein kinase 5 complex / G1 to G0 transition involved in cell differentiation / regulation of ubiquitin-protein transferase activity / epithelial cell maturation involved in prostate gland development / contractile muscle fiber / Activated NTRK2 signals through CDK5 / neuron cell-cell adhesion / layer formation in cerebral cortex / regulation of synaptic vesicle cycle ...superior olivary nucleus maturation / protein kinase 5 complex / G1 to G0 transition involved in cell differentiation / regulation of ubiquitin-protein transferase activity / epithelial cell maturation involved in prostate gland development / contractile muscle fiber / Activated NTRK2 signals through CDK5 / neuron cell-cell adhesion / layer formation in cerebral cortex / regulation of synaptic vesicle cycle / branching involved in prostate gland morphogenesis / regulation of dendritic spine morphogenesis / regulation of DNA damage checkpoint / ubiquitin-dependent protein catabolic process via the C-end degron rule pathway / CRMPs in Sema3A signaling / NGF-stimulated transcription / death receptor binding / regulation of extrinsic apoptotic signaling pathway via death domain receptors / cyclin-dependent protein serine/threonine kinase activator activity / axonal fasciculation / embryo development ending in birth or egg hatching / Cul2-RING ubiquitin ligase complex / regulation of neuron differentiation / Deregulated CDK5 triggers multiple neurodegenerative pathways in Alzheimer's disease models / beta-tubulin binding / protein kinase activator activity / regulation of cyclin-dependent protein serine/threonine kinase activity / alpha-tubulin binding / ubiquitin-like ligase-substrate adaptor activity / positive regulation of protein targeting to membrane / cyclin-dependent protein kinase holoenzyme complex / regulation of macroautophagy / ephrin receptor signaling pathway / positive regulation of microtubule polymerization / ionotropic glutamate receptor binding / NPAS4 regulates expression of target genes / ionotropic glutamate receptor signaling pathway / cerebellum development / protein serine/threonine kinase activator activity / ephrin receptor binding / axon guidance / hippocampus development / regulation of actin cytoskeleton organization / brain development / neuron migration / peptidyl-threonine phosphorylation / neuromuscular junction / G protein-coupled acetylcholine receptor signaling pathway / microtubule cytoskeleton organization / neuron differentiation / neuron projection development / positive regulation of neuron apoptotic process / rhythmic process / actin filament binding / presynapse / Neddylation / kinase activity / growth cone / perikaryon / proteasome-mediated ubiquitin-dependent protein catabolic process / peptidyl-serine phosphorylation / Regulation of TP53 Activity through Phosphorylation / protease binding / dendritic spine / postsynaptic density / protein ubiquitination / neuron projection / protein kinase activity / cadherin binding / axon / intracellular membrane-bounded organelle / negative regulation of DNA-templated transcription / dendrite / neuronal cell body / apoptotic process / calcium ion binding / protein kinase binding / perinuclear region of cytoplasm / nucleoplasm / membrane / metal ion binding / nucleus / plasma membrane / cytosol / cytoplasm Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.49 Å | ||||||
Authors | Chen, X. / Liao, S. / Xu, C. | ||||||
Funding support | China, 1items
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Citation | Journal: Nat.Chem.Biol. / Year: 2021 Title: Molecular basis for arginine C-terminal degron recognition by Cul2 FEM1 E3 ligase. Authors: Chen, X. / Liao, S. / Makaros, Y. / Guo, Q. / Zhu, Z. / Krizelman, R. / Dahan, K. / Tu, X. / Yao, X. / Koren, I. / Xu, C. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 7cng.cif.gz | 138.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb7cng.ent.gz | 106.6 KB | Display | PDB format |
PDBx/mmJSON format | 7cng.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/cn/7cng ftp://data.pdbj.org/pub/pdb/validation_reports/cn/7cng | HTTPS FTP |
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-Related structure data
Related structure data | 6lbfSC 6lbgC 6lbnC 6ldpC 6le6C 6lenC 6leyC 6lf0C S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 39295.809 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Details: FEM1B fused with CDK5R1 peptide, linked with linker residues GGGSGGGS. Source: (gene. exp.) Homo sapiens (human) / Gene: FEM1B, F1AA, KIAA0396, CDK5R1, CDK5R, NCK5A Production host: Escherichia coli 'BL21-Gold(DE3)pLysS AG' (bacteria) References: UniProt: Q9UK73, UniProt: Q15078 #2: Chemical | ChemComp-SO4 / Has ligand of interest | N | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.7 Å3/Da / Density % sol: 66.74 % |
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, sitting drop Details: 1.6M Magnesium sulfate hydrate, 0.1M BIS-TRIS propane pH 6.7 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: SSRF / Beamline: BL18U1 / Wavelength: 0.9791 Å |
Detector | Type: DECTRIS PILATUS3 S 6M / Detector: PIXEL / Date: Jul 20, 2020 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9791 Å / Relative weight: 1 |
Reflection | Resolution: 3.49→50 Å / Num. obs: 15530 / % possible obs: 100 % / Redundancy: 25.5 % / CC1/2: 1 / Rmerge(I) obs: 0.047 / Net I/σ(I): 24.5 |
Reflection shell | Resolution: 3.49→3.63 Å / Rmerge(I) obs: 2.271 / Num. unique obs: 1510 / CC1/2: 0.725 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 6LBF Resolution: 3.49→28.9 Å / SU ML: 0.44 / Cross valid method: THROUGHOUT / σ(F): 1.34 / Phase error: 25.7 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 103.08 Å2 / Biso mean: 51.3575 Å2 / Biso min: 18.85 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 3.49→28.9 Å
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0 / Total num. of bins used: 10
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