7THV
| Structure of the yeast clamp loader (Replication Factor C RFC) bound to the sliding clamp (Proliferating Cell Nuclear Antigen PCNA) in an autoinhibited conformation | Descriptor: | ADENOSINE-5'-DIPHOSPHATE, MAGNESIUM ION, PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER, ... | Authors: | Gaubitz, C, Liu, X, Pajak, J, Stone, N, Hayes, J, Demo, G, Kelch, B.A. | Deposit date: | 2022-01-12 | Release date: | 2022-02-16 | Last modified: | 2024-02-28 | Method: | ELECTRON MICROSCOPY (4 Å) | Cite: | Cryo-EM structures reveal high-resolution mechanism of a DNA polymerase sliding clamp loader. Elife, 11, 2022
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7TIB
| Structure of the yeast clamp loader (Replication Factor C RFC) bound to the open sliding clamp (Proliferating Cell Nuclear Antigen PCNA) and primer-template DNA | Descriptor: | ADENOSINE-5'-DIPHOSPHATE, DNA (5'-D(*AP*GP*AP*CP*AP*CP*TP*AP*CP*GP*AP*GP*TP*AP*CP*AP*TP*A)-3'), DNA (5'-D(P*TP*TP*TP*TP*TP*TP*TP*AP*TP*GP*TP*AP*CP*TP*CP*GP*TP*AP*GP*TP*GP*TP*CP*T)-3'), ... | Authors: | Gaubitz, C, Liu, X, Pajak, J, Stone, N, Hayes, J, Demo, G, Kelch, B.A. | Deposit date: | 2022-01-13 | Release date: | 2022-02-16 | Last modified: | 2024-02-28 | Method: | ELECTRON MICROSCOPY (3.4 Å) | Cite: | Cryo-EM structures reveal high-resolution mechanism of a DNA polymerase sliding clamp loader. Elife, 11, 2022
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7TIC
| Structure of the yeast clamp loader (Replication Factor C RFC) bound to the sliding clamp (Proliferating Cell Nuclear Antigen PCNA) in an autoinhibited conformation | Descriptor: | ADENOSINE-5'-DIPHOSPHATE, MAGNESIUM ION, PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER, ... | Authors: | Gaubitz, C, Liu, X, Pajak, J, Stone, N, Hayes, J, Demo, G, Kelch, B.A. | Deposit date: | 2022-01-13 | Release date: | 2022-02-16 | Last modified: | 2024-02-28 | Method: | ELECTRON MICROSCOPY (3.9 Å) | Cite: | Cryo-EM structures reveal high-resolution mechanism of a DNA polymerase sliding clamp loader. Elife, 11, 2022
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6O3H
| Icosahedral reconstruction of the thermophilic bacteriophage P74-26 capsid | Descriptor: | Major head protein, P74-26 Head Decoration Protein | Authors: | Stone, N.P, Demo, G, Agnello, E, Kelch, B.A. | Deposit date: | 2019-02-26 | Release date: | 2019-07-24 | Last modified: | 2024-03-20 | Method: | ELECTRON MICROSCOPY (2.8 Å) | Cite: | Principles for enhancing virus capsid capacity and stability from a thermophilic virus capsid structure. Nat Commun, 10, 2019
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8VAN
| Structure of the E. coli clamp loader bound to the beta clamp in an Initial-Binding conformation | Descriptor: | Beta sliding clamp, DNA polymerase III subunit delta, DNA polymerase III subunit delta', ... | Authors: | Landeck, J.T, Pajak, J, Kelch, B.A. | Deposit date: | 2023-12-11 | Release date: | 2024-03-27 | Last modified: | 2024-05-01 | Method: | ELECTRON MICROSCOPY (7.7 Å) | Cite: | Differences between bacteria and eukaryotes in clamp loader mechanism, a conserved process underlying DNA replication. J.Biol.Chem., 300, 2024
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7U1A
| RFC:PCNA bound to dsDNA with a ssDNA gap of six nucleotides | Descriptor: | ADENOSINE-5'-DIPHOSPHATE, DNA - Primer, DNA - Template, ... | Authors: | Liu, X, Gaubitz, C, Pajak, J, Kelch, B.A. | Deposit date: | 2022-02-20 | Release date: | 2022-07-06 | Last modified: | 2024-02-21 | Method: | ELECTRON MICROSCOPY (3.3 Å) | Cite: | A second DNA binding site on RFC facilitates clamp loading at gapped or nicked DNA. Elife, 11, 2022
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7U1P
| RFC:PCNA bound to DNA with a ssDNA gap of five nucleotides | Descriptor: | ADENOSINE-5'-DIPHOSPHATE, DNA - Primer, DNA - Template, ... | Authors: | Liu, X, Gaubitz, C, Pajak, J, Kelch, B.A. | Deposit date: | 2022-02-21 | Release date: | 2022-07-06 | Last modified: | 2024-02-21 | Method: | ELECTRON MICROSCOPY (3 Å) | Cite: | A second DNA binding site on RFC facilitates clamp loading at gapped or nicked DNA. Elife, 11, 2022
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7U19
| RFC:PCNA bound to nicked DNA | Descriptor: | ADENOSINE-5'-DIPHOSPHATE, DNA, MAGNESIUM ION, ... | Authors: | Liu, X, Gaubitz, C, Pajak, J, Kelch, B.A. | Deposit date: | 2022-02-20 | Release date: | 2022-07-06 | Last modified: | 2024-02-21 | Method: | ELECTRON MICROSCOPY (3.7 Å) | Cite: | A second DNA binding site on RFC facilitates clamp loading at gapped or nicked DNA. Elife, 11, 2022
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6BL5
| Head decoration protein from the hyperthermophilic phage P74-26 | Descriptor: | Head decoration protein | Authors: | Stone, N.P, Hilbert, B.J, Hidalgo, D, Halloran, K.T, Kelch, B.A. | Deposit date: | 2017-11-09 | Release date: | 2018-02-21 | Last modified: | 2024-03-13 | Method: | X-RAY DIFFRACTION (1.69 Å) | Cite: | A Hyperthermophilic Phage Decoration Protein Suggests Common Evolutionary Origin with Herpesvirus Triplex Proteins and an Anti-CRISPR Protein. Structure, 26, 2018
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5TGE
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4ZNL
| Thermus Phage P74-26 Large Terminase ATPase domain bound to ADP Beryllium Fluoride | Descriptor: | ADENOSINE-5'-DIPHOSPHATE, BERYLLIUM TRIFLUORIDE ION, MAGNESIUM ION, ... | Authors: | Hilbert, B.J, Hayes, J.A, Stone, N.P, Duffy, C.M, Kelch, B.A. | Deposit date: | 2015-05-04 | Release date: | 2015-07-08 | Last modified: | 2023-09-27 | Method: | X-RAY DIFFRACTION (2.068 Å) | Cite: | Structure and mechanism of the ATPase that powers viral genome packaging. Proc.Natl.Acad.Sci.USA, 112, 2015
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6V1I
| Cryo-EM reconstruction of the thermophilic bacteriophage P74-26 small terminase- symmetric | Descriptor: | Small terminase protein | Authors: | Hayes, J.A, Hilbert, B.J, Gaubitz, C, Stone, N.P, Kelch, B.A. | Deposit date: | 2019-11-20 | Release date: | 2020-02-12 | Last modified: | 2024-03-06 | Method: | ELECTRON MICROSCOPY (3.8 Å) | Cite: | A thermophilic phage uses a small terminase protein with a fixed helix-turn-helix geometry. J.Biol.Chem., 295, 2020
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6VVO
| Structure of the human clamp loader (Replication Factor C, RFC) bound to the sliding clamp (Proliferating Cell Nuclear Antigen, PCNA) | Descriptor: | ADENOSINE-5'-DIPHOSPHATE, MAGNESIUM ION, PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER, ... | Authors: | Gaubitz, C, Liu, X, Stone, N.P, Kelch, B.A. | Deposit date: | 2020-02-18 | Release date: | 2020-02-26 | Last modified: | 2020-03-25 | Method: | ELECTRON MICROSCOPY (3.4 Å) | Cite: | Structure of the human clamp loader bound to the sliding clamp: a further twist on AAA+ mechanism Biorxiv, 2020
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4ZNI
| Thermus Phage P74-26 Large Terminase ATPase domain (I 2 3 space group) | Descriptor: | Phage terminase large subunit, SULFATE ION | Authors: | Hilbert, B.J, Hayes, J.A, Stone, N.P, Duffy, C.M, Sankaran, B, Kelch, B.A. | Deposit date: | 2015-05-04 | Release date: | 2015-07-08 | Last modified: | 2024-03-06 | Method: | X-RAY DIFFRACTION (2.097 Å) | Cite: | Structure and mechanism of the ATPase that powers viral genome packaging. Proc.Natl.Acad.Sci.USA, 112, 2015
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4ZNJ
| Thermus Phage P74-26 Large Terminase ATPase domain mutant R139A (I 2 3 space group) | Descriptor: | Phage terminase large subunit, SULFATE ION | Authors: | Hilbert, B.J, Hayes, J.A, Stone, N.P, Duffy, C.M, Kelch, B.A. | Deposit date: | 2015-05-04 | Release date: | 2015-07-08 | Last modified: | 2023-09-27 | Method: | X-RAY DIFFRACTION (2.532 Å) | Cite: | Structure and mechanism of the ATPase that powers viral genome packaging. Proc.Natl.Acad.Sci.USA, 112, 2015
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4ZNK
| Thermus Phage P74-26 Large Terminase ATPase domain from (P 32 2 1 space group) | Descriptor: | Phage terminase large subunit, SULFATE ION | Authors: | Hilbert, B.J, Hayes, J.A, Stone, N.P, Duffy, C.M, Kelch, B.A. | Deposit date: | 2015-05-04 | Release date: | 2015-07-08 | Last modified: | 2023-09-27 | Method: | X-RAY DIFFRACTION (1.931 Å) | Cite: | Structure and mechanism of the ATPase that powers viral genome packaging. Proc.Natl.Acad.Sci.USA, 112, 2015
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5E0V
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5E0T
| Human PCNA mutant - S228I | Descriptor: | Proliferating cell nuclear antigen | Authors: | Duffy, C.M, Hilbert, B.J, Kelch, B.A. | Deposit date: | 2015-09-29 | Release date: | 2016-04-20 | Last modified: | 2023-09-27 | Method: | X-RAY DIFFRACTION (2.6653 Å) | Cite: | A Disease-Causing Variant in PCNA Disrupts a Promiscuous Protein Binding Site. J.Mol.Biol., 428, 2016
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4JOM
| Structure of E. coli Pol III 3mPHP mutant | Descriptor: | DNA polymerase III subunit alpha, GLYCEROL, PHOSPHATE ION, ... | Authors: | Barros, T, Guenther, J, Kelch, B, Anaya, J, Prabhakar, A, O'Donnell, M, Kuriyan, J, Lamers, M.H. | Deposit date: | 2013-03-18 | Release date: | 2013-05-29 | Last modified: | 2023-09-20 | Method: | X-RAY DIFFRACTION (2.9 Å) | Cite: | A structural role for the PHP domain in E. coli DNA polymerase III. Bmc Struct.Biol., 13, 2013
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5E0U
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8UH7
| Structure of T4 Bacteriophage clamp loader bound to the T4 clamp, primer-template DNA, and ATP analog | Descriptor: | ADENOSINE-5'-DIPHOSPHATE, MAGNESIUM ION, Primer DNA strand, ... | Authors: | Gee, C.L, Marcus, K, Kelch, B.A, Makino, D.L. | Deposit date: | 2023-10-07 | Release date: | 2023-12-13 | Last modified: | 2024-04-03 | Method: | X-RAY DIFFRACTION (2.628 Å) | Cite: | Autoinhibition of a clamp-loader ATPase revealed by deep mutagenesis and cryo-EM. Nat.Struct.Mol.Biol., 31, 2024
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