6BZO
 
 | | Mtb RNAP Holo/RbpA/Fidaxomicin/upstream fork DNA | | Descriptor: | DNA (26-MER), DNA (32-MER), DNA-directed RNA polymerase subunit alpha, ... | | Authors: | Darst, S.A, Campbell, E.A, Boyaci Selcuk, H, Chen, J. | | Deposit date: | 2017-12-25 | | Release date: | 2018-03-28 | | Last modified: | 2024-03-13 | | Method: | ELECTRON MICROSCOPY (3.38 Å) | | Cite: | Fidaxomicin jamsMycobacterium tuberculosisRNA polymerase motions needed for initiation via RbpA contacts.
Elife, 7, 2018
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3D36
 | | How to Switch Off a Histidine Kinase: Crystal Structure of Geobacillus stearothermophilus KinB with the Inhibitor Sda | | Descriptor: | (4S)-2-METHYL-2,4-PENTANEDIOL, ADENOSINE-5'-DIPHOSPHATE, MAGNESIUM ION, ... | | Authors: | Bick, M.J, Lamour, V, Rajashankar, K.R, Gordiyenko, Y, Robinson, C.V, Darst, S.A. | | Deposit date: | 2008-05-09 | | Release date: | 2009-01-13 | | Last modified: | 2024-02-21 | | Method: | X-RAY DIFFRACTION (2.03 Å) | | Cite: | How to switch off a histidine kinase: crystal structure of Geobacillus stearothermophilus KinB with the inhibitor Sda
J.Mol.Biol., 386, 2009
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2GHO
 | | Recombinant Thermus aquaticus RNA polymerase for Structural Studies | | Descriptor: | DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, DNA-directed RNA polymerase subunit beta',DNA-directed RNA polymerase subunit beta' | | Authors: | Lamour, V, Darst, S.A. | | Deposit date: | 2006-03-27 | | Release date: | 2006-05-23 | | Last modified: | 2024-02-14 | | Method: | X-RAY DIFFRACTION (5 Å) | | Cite: | Recombinant Thermus aquaticus RNA Polymerase for Structural Studies.
J.Mol.Biol., 359, 2006
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3MGC
 | | Teg12 Apo | | Descriptor: | GLYCEROL, IMIDAZOLE, N-L-ALPHA-ASPARTYL L-PHENYLALANINE 1-METHYL ESTER, ... | | Authors: | Bick, M.J, Banik, J.J, Darst, S.A, Brady, S.F. | | Deposit date: | 2010-04-05 | | Release date: | 2010-06-09 | | Last modified: | 2023-09-06 | | Method: | X-RAY DIFFRACTION (2.91 Å) | | Cite: | Crystal structures of the glycopeptide sulfotransferase Teg12 in a complex with the teicoplanin aglycone.
Biochemistry, 49, 2010
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3MG9
 | | Teg 12 Binary Structure Complexed with the Teicoplanin Aglycone | | Descriptor: | FORMIC ACID, GLYCEROL, TEG12, ... | | Authors: | Bick, M.J, Banik, J.J, Darst, S.A, Brady, S.F. | | Deposit date: | 2010-04-05 | | Release date: | 2010-06-09 | | Last modified: | 2023-11-22 | | Method: | X-RAY DIFFRACTION (2.27 Å) | | Cite: | Crystal Structures of the Glycopeptide Sulfotransferase Teg12 in a Complex with the Teicoplanin Aglycone.
Biochemistry, 49, 2010
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1HQM
 | | CRYSTAL STRUCTURE OF THERMUS AQUATICUS CORE RNA POLYMERASE-INCLUDES COMPLETE STRUCTURE WITH SIDE-CHAINS (EXCEPT FOR DISORDERED REGIONS)-FURTHER REFINED FROM ORIGINAL DEPOSITION-CONTAINS ADDITIONAL SEQUENCE INFORMATION | | Descriptor: | DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, DNA-directed RNA polymerase subunit beta', ... | | Authors: | Minakhin, L, Bhagat, S, Brunning, A, Campbell, E.A, Darst, S.A, Ebright, R.H, Severinov, K. | | Deposit date: | 2000-12-18 | | Release date: | 2001-02-07 | | Last modified: | 2024-02-07 | | Method: | X-RAY DIFFRACTION (3.3 Å) | | Cite: | Bacterial RNA polymerase subunit omega and eukaryotic RNA polymerase subunit RPB6 are sequence, structural, and functional homologs and promote RNA polymerase assembly.
Proc.Natl.Acad.Sci.USA, 98, 2001
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2AUJ
 | | Structure of Thermus aquaticus RNA polymerase beta'-subunit insert | | Descriptor: | DNA-directed RNA polymerase beta' chain | | Authors: | Chlenov, M, Masuda, S, Murakami, K.S, Nikiforov, V, Darst, S.A, Mustaev, A. | | Deposit date: | 2005-08-27 | | Release date: | 2005-10-04 | | Last modified: | 2024-02-14 | | Method: | X-RAY DIFFRACTION (2.7 Å) | | Cite: | Structure and Function of Lineage-specific Sequence Insertions in the Bacterial RNA Polymerase beta' Subunit
J.Mol.Biol., 353, 2005
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2AUK
 | | Structure of E. coli RNA polymerase beta' G/G' insert | | Descriptor: | DNA-directed RNA polymerase beta' chain | | Authors: | Chlenov, M, Masuda, S, Murakami, K.S, Nikiforov, V, Darst, S.A, Mustaev, A. | | Deposit date: | 2005-08-28 | | Release date: | 2005-10-04 | | Last modified: | 2024-02-14 | | Method: | X-RAY DIFFRACTION (2.3 Å) | | Cite: | Structure and Function of Lineage-specific Sequence Insertions in the Bacterial RNA Polymerase beta' Subunit
J.Mol.Biol., 353, 2005
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2EYQ
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2ETN
 | | Crystal structure of Thermus aquaticus Gfh1 | | Descriptor: | anti-cleavage anti-GreA transcription factor Gfh1 | | Authors: | Lamour, V, Hogan, B.P, Erie, D.A, Darst, S.A. | | Deposit date: | 2005-10-27 | | Release date: | 2006-02-14 | | Last modified: | 2024-02-14 | | Method: | X-RAY DIFFRACTION (3.3 Å) | | Cite: | Crystal structure of Thermus aquaticus Gfh1, a Gre-factor paralog that inhibits rather than stimulates transcript cleavage.
J.Mol.Biol., 356, 2006
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8SQ9
 | | SARS-CoV-2 replication-transcription complex bound to nsp9 and UMPCPP, as a pre-catalytic NMPylation intermediate | | Descriptor: | 5'-O-[(S)-hydroxy{[(S)-hydroxy(phosphonooxy)phosphoryl]methyl}phosphoryl]uridine, MAGNESIUM ION, Non-structural protein 7, ... | | Authors: | Small, G.I, Darst, S.A, Campbell, E.A. | | Deposit date: | 2023-05-04 | | Release date: | 2023-11-22 | | Method: | ELECTRON MICROSCOPY (2.9 Å) | | Cite: | Structural and functional insights into the enzymatic plasticity of the SARS-CoV-2 NiRAN domain.
Mol.Cell, 83, 2023
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8SQK
 | | SARS-CoV-2 replication-transcription complex bound to RNA-nsp9 and GDP-betaS, as a pre-catalytic deRNAylation/mRNA capping intermediate | | Descriptor: | 5'-O-[(R)-hydroxy(thiophosphonooxy)phosphoryl]guanosine, MAGNESIUM ION, Non-structural protein 7, ... | | Authors: | Small, G.I, Darst, S.A, Campbell, E.A. | | Deposit date: | 2023-05-04 | | Release date: | 2023-11-22 | | Method: | ELECTRON MICROSCOPY (3.01 Å) | | Cite: | Structural and functional insights into the enzymatic plasticity of the SARS-CoV-2 NiRAN domain.
Mol.Cell, 83, 2023
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8SQJ
 | | SARS-CoV-2 replication-transcription complex bound to RNA-nsp9, as a noncatalytic RNA-nsp9 binding mode | | Descriptor: | 5'-O-[(R)-hydroxy(thiophosphonooxy)phosphoryl]guanosine, MAGNESIUM ION, Non-structural protein 7, ... | | Authors: | Small, G.I, Darst, S.A, Campbell, E.A. | | Deposit date: | 2023-05-04 | | Release date: | 2023-11-22 | | Method: | ELECTRON MICROSCOPY (3.06 Å) | | Cite: | Structural and functional insights into the enzymatic plasticity of the SARS-CoV-2 NiRAN domain.
Mol.Cell, 83, 2023
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6N62
 | | Escherichia coli RNA polymerase sigma70-holoenzyme bound to upstream fork promoter DNA | | Descriptor: | DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, DNA-directed RNA polymerase subunit beta', ... | | Authors: | Braffman, N, Hauver, J, Campbell, E.A, Darst, S.A. | | Deposit date: | 2018-11-24 | | Release date: | 2019-01-09 | | Last modified: | 2023-10-11 | | Method: | X-RAY DIFFRACTION (3.803 Å) | | Cite: | Structural mechanism of transcription inhibition by lasso peptides microcin J25 and capistruin.
Proc. Natl. Acad. Sci. U.S.A., 116, 2019
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6N61
 | | Escherichia coli RNA polymerase sigma70-holoenzyme bound to upstream fork promoter DNA and Capistruin | | Descriptor: | 1,2-ETHANEDIOL, 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID, Capistruin, ... | | Authors: | Braffman, N, Hauver, J, Campbell, E.A, Darst, S.A. | | Deposit date: | 2018-11-24 | | Release date: | 2019-01-09 | | Last modified: | 2023-10-11 | | Method: | X-RAY DIFFRACTION (3.253 Å) | | Cite: | Structural mechanism of transcription inhibition by lasso peptides microcin J25 and capistruin.
Proc. Natl. Acad. Sci. U.S.A., 116, 2019
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6N57
 | | Cryo-EM structure of Escherichia coli RNAP polymerase bound with TraR in conformation I | | Descriptor: | CHAPSO, DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, ... | | Authors: | Chen, J, Chiu, C.E, Campbell, E.A, Darst, S.A. | | Deposit date: | 2018-11-21 | | Release date: | 2020-02-26 | | Last modified: | 2024-03-20 | | Method: | ELECTRON MICROSCOPY (3.7 Å) | | Cite: | E. coliTraR allosterically regulates transcription initiation by altering RNA polymerase conformation.
Elife, 8, 2019
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6N58
 | | Cryo-EM structure of Escherichia coli RNAP polymerase bound with TraR in conformation II | | Descriptor: | CHAPSO, DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, ... | | Authors: | Chen, J, Chiu, C.E, Campbell, E.A, Darst, S.A. | | Deposit date: | 2018-11-21 | | Release date: | 2020-02-26 | | Last modified: | 2024-03-20 | | Method: | ELECTRON MICROSCOPY (3.78 Å) | | Cite: | E. coliTraR allosterically regulates transcription initiation by altering RNA polymerase conformation.
Elife, 8, 2019
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6N60
 | | Escherichia coli RNA polymerase sigma70-holoenzyme bound to upstream fork promoter DNA and Microcin J25 (MccJ25) | | Descriptor: | DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, DNA-directed RNA polymerase subunit beta', ... | | Authors: | Braffman, N, Hauver, J, Campbell, E.A, Darst, S.A. | | Deposit date: | 2018-11-23 | | Release date: | 2019-01-09 | | Last modified: | 2023-10-11 | | Method: | X-RAY DIFFRACTION (3.68 Å) | | Cite: | Structural mechanism of transcription inhibition by lasso peptides microcin J25 and capistruin.
Proc. Natl. Acad. Sci. U.S.A., 116, 2019
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6OMF
 | | CryoEM structure of SigmaS-transcription initiation complex with activator Crl | | Descriptor: | DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, DNA-directed RNA polymerase subunit beta', ... | | Authors: | Jaramillo Cartagena, A, Darst, S.A, Campbell, E.A. | | Deposit date: | 2019-04-18 | | Release date: | 2019-08-28 | | Last modified: | 2024-03-13 | | Method: | ELECTRON MICROSCOPY (3.26 Å) | | Cite: | Structural basis for transcription activation by Crl through tethering of sigmaSand RNA polymerase.
Proc.Natl.Acad.Sci.USA, 116, 2019
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1YNN
 | | Taq RNA polymerase-rifampicin complex | | Descriptor: | DNA-directed RNA polymerase alpha chain, DNA-directed RNA polymerase beta chain, DNA-directed RNA polymerase beta' chain, ... | | Authors: | Campbell, E.A, Pavlova, O, Zenkin, N, Leon, F, Irschik, H, Jansen, R, Severinov, K, Darst, S.A. | | Deposit date: | 2005-01-24 | | Release date: | 2005-03-15 | | Last modified: | 2024-02-14 | | Method: | X-RAY DIFFRACTION (3.3 Å) | | Cite: | Structural, functional, and genetic analysis of sorangicin inhibition of bacterial RNA polymerase
Embo J., 24, 2005
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7RDZ
 | | SARS-CoV-2 replication-transcription complex bound to nsp13 helicase - nsp13(2)-RTC - apo class | | Descriptor: | ADENOSINE-5'-DIPHOSPHATE, Helicase, MAGNESIUM ION, ... | | Authors: | Chen, J, Malone, B, Campbell, E.A, Darst, S.A. | | Deposit date: | 2021-07-12 | | Release date: | 2021-11-24 | | Last modified: | 2024-06-05 | | Method: | ELECTRON MICROSCOPY (3.6 Å) | | Cite: | Ensemble cryo-EM reveals conformational states of the nsp13 helicase in the SARS-CoV-2 helicase replication-transcription complex.
Nat.Struct.Mol.Biol., 29, 2022
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7RDX
 | | SARS-CoV-2 replication-transcription complex bound to nsp13 helicase - nsp13(2)-RTC - open class | | Descriptor: | ADENOSINE-5'-DIPHOSPHATE, ALUMINUM FLUORIDE, CHAPSO, ... | | Authors: | Chen, J, Malone, B, Campbell, E.A, Darst, S.A. | | Deposit date: | 2021-07-12 | | Release date: | 2021-11-24 | | Last modified: | 2024-06-05 | | Method: | ELECTRON MICROSCOPY (3.1 Å) | | Cite: | Ensemble cryo-EM reveals conformational states of the nsp13 helicase in the SARS-CoV-2 helicase replication-transcription complex.
Nat.Struct.Mol.Biol., 29, 2022
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1OR7
 | | Crystal Structure of Escherichia coli sigmaE with the Cytoplasmic Domain of its Anti-sigma RseA | | Descriptor: | RNA polymerase sigma-E factor, Sigma-E factor negative regulatory protein | | Authors: | Campbell, E.A, Tupy, J.L, Gruber, T.M, Wang, S, Sharp, M.M, Gross, C.A, Darst, S.A. | | Deposit date: | 2003-03-12 | | Release date: | 2003-04-15 | | Last modified: | 2024-02-14 | | Method: | X-RAY DIFFRACTION (2 Å) | | Cite: | Crystal structure of Escherichia coli sigmaE with the cytoplasmic domain of its anti-sigma RseA.
Mol.Cell, 11, 2003
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3GFK
 | | Crystal structure of Bacillus subtilis Spx/RNA polymerase alpha subunit C-terminal domain complex | | Descriptor: | DNA-directed RNA polymerase subunit alpha, Regulatory protein spx | | Authors: | Lamour, V, Westblade, L.F, Campbell, E.A, Darst, S.A. | | Deposit date: | 2009-02-27 | | Release date: | 2009-03-10 | | Last modified: | 2023-09-06 | | Method: | X-RAY DIFFRACTION (2.3 Å) | | Cite: | Crystal structure of the in vivo-assembled Bacillus subtilis Spx/RNA polymerase alpha subunit C-terminal domain complex
J.Struct.Biol., 168, 2009
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7RE3
 | | SARS-CoV-2 replication-transcription complex bound to nsp13 helicase - nsp13(2)-RTC dimer | | Descriptor: | ADENOSINE-5'-DIPHOSPHATE, ALUMINUM FLUORIDE, CHAPSO, ... | | Authors: | Chen, J, Malone, B, Campbell, E.A, Darst, S.A. | | Deposit date: | 2021-07-12 | | Release date: | 2021-11-24 | | Last modified: | 2024-06-05 | | Method: | ELECTRON MICROSCOPY (3.33 Å) | | Cite: | Ensemble cryo-EM reveals conformational states of the nsp13 helicase in the SARS-CoV-2 helicase replication-transcription complex.
Nat.Struct.Mol.Biol., 29, 2022
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