6N60

Escherichia coli RNA polymerase sigma70-holoenzyme bound to upstream fork promoter DNA and Microcin J25 (MccJ25)

Summary for 6N60

Related PRD IDPRD_000184
DescriptorDNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, DNA-directed RNA polymerase subunit beta', ... (10 entities in total)
Functional Keywordscomplex, inhibitor, lasso peptide, microcin j25, rna polymerase, transferase-dna complex, transferase/dna
Biological sourceEscherichia coli
Total number of polymer chains9
Total molecular weight458373.76
Authors
Braffman, N.,Hauver, J.,Campbell, E.A.,Darst, S.A. (deposition date: 2018-11-23, release date: 2019-01-09, Last modification date: 2020-01-01)
Primary citation
Braffman, N.R.,Piscotta, F.J.,Hauver, J.,Campbell, E.A.,Link, A.J.,Darst, S.A.
Structural mechanism of transcription inhibition by lasso peptides microcin J25 and capistruin.
Proc. Natl. Acad. Sci. U.S.A., 116:1273-1278, 2019
PubMed: 30626643 (PDB entries with the same primary citation)
DOI: 10.1073/pnas.1817352116
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (3.68 Å)
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers 0.31615 0.5% 11.7% 3.6%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution
Download full validation reportDownload
171313
PDB entries from 2020-11-18