6N60
Escherichia coli RNA polymerase sigma70-holoenzyme bound to upstream fork promoter DNA and Microcin J25 (MccJ25)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-07-10 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.97630 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 172.906, 172.906, 387.263 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.551 - 3.680 |
R-factor | 0.2647 |
Rwork | 0.263 |
R-free | 0.30620 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ljz |
RMSD bond length | 0.003 |
RMSD bond angle | 0.684 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.810 |
High resolution limit [Å] | 3.680 | 3.680 |
Rpim | 0.060 | 1.390 |
Number of reflections | 64026 | 5759 |
<I/σ(I)> | 10.14 | 0.55 |
Completeness [%] | 98.0 | 91 |
Redundancy | 9.2 | 4.3 |
CC(1/2) | 0.144 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 295 | 0.1 M HEPES, pH 6.7, 0.2 M magnesium chloride, 7% w/v PEG3350, 4% v/v glycerol |