6N60

Escherichia coli RNA polymerase sigma70-holoenzyme bound to upstream fork promoter DNA and Microcin J25 (MccJ25)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-07-10
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97630
Spacegroup name	P 41 21 2
Unit cell lengths	172.906, 172.906, 387.263
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.551 - 3.680
R-factor	0.2647
Rwork	0.263
R-free	0.30620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ljz
RMSD bond length	0.003
RMSD bond angle	0.684
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.810
High resolution limit [Å]	3.680	3.680
Rpim	0.060	1.390
Number of reflections	64026	5759
<I/σ(I)>	10.14	0.55
Completeness [%]	98.0	91
Redundancy	9.2	4.3
CC(1/2)		0.144

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.8	295	0.1 M HEPES, pH 6.7, 0.2 M magnesium chloride, 7% w/v PEG3350, 4% v/v glycerol