2AUJ

Structure of Thermus aquaticus RNA polymerase beta'-subunit insert

Summary for 2AUJ

• Entry DOI: 10.2210/pdb2auj/pdb
• Related: 2AUK
• Descriptor: DNA-directed RNA polymerase beta' chain (1 entity in total)
• Functional Keywords: sandwich-barrel hybrid motif, transferase
• Biological source: Thermus aquaticus
• Total number of polymer chains: 1
• Total formula weight: 34489.10

Authors

Chlenov, M.,Masuda, S.,Murakami, K.S.,Nikiforov, V.,Darst, S.A.,Mustaev, A. (deposition date: 2005-08-27, release date: 2005-10-04, Last modification date: 2024-02-14)

Primary citation

Chlenov, M.,Masuda, S.,Murakami, K.S.,Nikiforov, V.,Darst, S.A.,Mustaev, A.
Structure and Function of Lineage-specific Sequence Insertions in the Bacterial RNA Polymerase beta' Subunit
J.Mol.Biol., 353:138-154, 2005

PubMed Abstract: The large beta and beta' subunits of the bacterial core RNA polymerase (RNAP) are highly conserved throughout evolution. Nevertheless, large sequence insertions in beta and beta' characterize specific evolutionary lineages of bacteria. The Thermus aquaticus RNAP beta' subunit contains a 283 residue insert between conserved regions A and B that is found in only four bacterial species. The Escherichia coli RNAP beta' subunit contains a 188 residue insert in the middle of conserved region G that is found in a wide range of bacterial species. Here, we present structural studies of these two beta' insertions. We show that the inserts comprise repeats of a previously characterized fold, the sandwich-barrel hybrid motif (as predicted from previous sequence analysis) and that the inserts serve significant roles in facilitating protein/protein and/or protein/nucleic acid interactions.

PubMed: 16154587
DOI: 10.1016/j.jmb.2005.07.073

Experimental method

X-RAY DIFFRACTION (2.7 Å)