2AUJ

Structure of Thermus aquaticus RNA polymerase beta'-subunit insert

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Detector technology	CCD
Collection date	2000-08-25
Wavelength(s)	0.92511
Spacegroup name	P 1 21 1
Unit cell lengths	59.724, 53.649, 69.693
Unit cell angles	90.00, 107.09, 90.00

Refinement procedure

Resolution	35.000 - 2.700
R-factor	0.236
Rwork	0.236
R-free	0.27900
Structure solution method	combination of molecular replacement and SAD
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	35.000
High resolution limit [Å]	2.700
Number of reflections	10739

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	sodium cacodylate/magnesium acetate/polyethylene glycol 8000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K