3GFK
Crystal structure of Bacillus subtilis Spx/RNA polymerase alpha subunit C-terminal domain complex
Summary for 3GFK
| Entry DOI | 10.2210/pdb3gfk/pdb |
| Descriptor | Regulatory protein spx, DNA-directed RNA polymerase subunit alpha (3 entities in total) |
| Functional Keywords | protein-protein complex, cytoplasm, redox-active center, stress response, transcription, transcription regulation, dna-directed rna polymerase, nucleotidyltransferase, transferase, transcription-transferase complex, transcription/transferase |
| Biological source | Bacillus subtilis More |
| Cellular location | Cytoplasm (Probable): O31602 |
| Total number of polymer chains | 2 |
| Total formula weight | 24670.36 |
| Authors | Lamour, V.,Westblade, L.F.,Campbell, E.A.,Darst, S.A. (deposition date: 2009-02-27, release date: 2009-03-10, Last modification date: 2024-10-30) |
| Primary citation | Lamour, V.,Westblade, L.F.,Campbell, E.A.,Darst, S.A. Crystal structure of the in vivo-assembled Bacillus subtilis Spx/RNA polymerase alpha subunit C-terminal domain complex J.Struct.Biol., 168:352-356, 2009 Cited by PubMed Abstract: The Bacillus subtilis Spx protein is a global transcription factor that interacts with the C-terminal domain of the RNA polymerase alpha subunit (alphaCTD) and regulates transcription of genes involved in thiol-oxidative stress, sporulation, competence, and organosulfur metabolism. Here we determined the X-ray crystal structure of the Spx/alphaCTD complex from an entirely new crystal form than previously reported [Newberry, K.J., Nakano, S., Zuber, P., Brennan, R.G., 2005. Crystal structure of the Bacillus subtilis anti-alpha, global transcriptional regulator, Spx, in complex with the alpha C-terminal domain of RNA polymerase. Proc. Natl. Acad. Sci. USA 102, 15839-15844]. Comparison of the previously reported sulfate-bound complex and our sulfate-free complex reveals subtle conformational changes that may be important for the role of Spx in regulating organosulfur metabolism. PubMed: 19580872DOI: 10.1016/j.jsb.2009.07.001 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.3 Å) |
Structure validation
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