7JPE

Room Temperature Structure of SARS-CoV-2 Nsp10/Nsp16 Methyltransferase in a Complex with m7GpppA Cap-0 and SAM Determined by Fixed-Target Serial Crystallography

7JPE の概要

• エントリーDOI: 10.2210/pdb7jpe/pdb
• 関連するPDBエントリー: 6W4H 6WJT 6WKQ 6WQ3 6WRZ 6WVN 6XKM 7JHE 7JIB
• 分子名称: 2'-O-methyltransferase, Non-structural protein 10, P1-7-METHYLGUANOSINE-P3-ADENOSINE-5',5'-TRIPHOSPHATE, ... (7 entities in total)
• 機能のキーワード: sars cov-2, serial crystallography, methyltransferase, m7gpppa, cap-0, s-adenosylhomomethionine, structural genomics, center for structural genomics of infectious diseases, csgid, viral protein
• 由来する生物種: Severe acute respiratory syndrome coronavirus 2 (2019-nCoV); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 50477.63

構造登録者

Wilamowski, M.,Sherrell, D.A.,Minasov, G.,Kim, Y.,Shuvalova, L.,Lavens, A.,Chard, R.,Rosas-Lemus, M.,Maltseva, N.,Jedrzejczak, R.,Michalska, K.,Satchell, K.J.F.,Joachimiak, A.,Center for Structural Genomics of Infectious Diseases (CSGID) (登録日: 2020-08-07, 公開日: 2020-08-26, 最終更新日: 2024-02-28)

主引用文献

Wilamowski, M.,Sherrell, D.A.,Minasov, G.,Kim, Y.,Shuvalova, L.,Lavens, A.,Chard, R.,Maltseva, N.,Jedrzejczak, R.,Rosas-Lemus, M.,Saint, N.,Foster, I.T.,Michalska, K.,Satchell, K.J.F.,Joachimiak, A.
2'-O methylation of RNA cap in SARS-CoV-2 captured by serial crystallography.
Proc.Natl.Acad.Sci.USA, 118:-, 2021

PubMed Abstract: The genome of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) coronavirus has a capping modification at the 5'-untranslated region (UTR) to prevent its degradation by host nucleases. These modifications are performed by the Nsp10/14 and Nsp10/16 heterodimers using S-adenosylmethionine as the methyl donor. Nsp10/16 heterodimer is responsible for the methylation at the ribose 2'-O position of the first nucleotide. To investigate the conformational changes of the complex during 2'-O methyltransferase activity, we used a fixed-target serial synchrotron crystallography method at room temperature. We determined crystal structures of Nsp10/16 with substrates and products that revealed the states before and after methylation, occurring within the crystals during the experiments. Here we report the crystal structure of Nsp10/16 in complex with Cap-1 analog (GpppA). Inhibition of Nsp16 activity may reduce viral proliferation, making this protein an attractive drug target.

PubMed: 33972410
DOI: 10.1073/pnas.2100170118

実験手法

X-RAY DIFFRACTION (2.18 Å)