7JPE

Room Temperature Structure of SARS-CoV-2 Nsp10/Nsp16 Methyltransferase in a Complex with m7GpppA Cap-0 and SAM Determined by Fixed-Target Serial Crystallography

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	2'-O-methyltransferase	polymer	301	33627.5	1	UniProt (P0DTD1) Pfam (PF06460)	Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)	Non-structural protein 16
2	B (B)	Non-structural protein 10	polymer	142	15075.2	1	UniProt (P0DTD1) Pfam (PF09401)	Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
3	C (A)	P1-7-METHYLGUANOSINE-P3-ADENOSINE-5',5'-TRIPHOSPHATE	non-polymer		787.4	1	Chemie (GTA)
4	D (A)	S-ADENOSYLMETHIONINE	non-polymer		398.4	1	Chemie (SAM) PubChem (58766358) PubChem (87224684) PubChem (131697735) PubChem (145712080) PubChem (58633578) PubChem (60038769) PubChem (73897310) PubChem (5136) PubChem (34755) PubChem (9865603) PubChem (12312798) PubChem (132988922) PubChem (134692588) PubChem (135030730) PubChem (140459785) PubChem (163046094)
5	E (A)	7N-METHYL-8-HYDROGUANOSINE-5'-DIPHOSPHATE	non-polymer		458.2	1	Chemie (M7G)
6	F, G (B)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
7	H, I (A, B)	water	water		18.0	127	Chemie (HOH)

Sequence modifications

A: 1 - 298 (UniProt: P0DTD1)

PDB	External Database	Details
Ser -2	-	expression tag
Asn -1	-	expression tag
Ala 0	-	expression tag

B: 1 - 139 (UniProt: P0DTD1)

PDB	External Database	Details
Ser -2	-	expression tag
Asn -1	-	expression tag
Ala 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	48702.7
Non-Polymers*	Number of molecules	5
	Total formula weight	1774.9
All*	Total formula weight	50477.6

*Water molecules are not included.