5SVS
Anomalous Mn2+ signal reveals a divalent cation-binding site in the head domain of the ATP-gated human P2X3 ion channel
Summary for 5SVS
Entry DOI | 10.2210/pdb5svs/pdb |
Related | 5SVJ 5SVK 5SVL 5SVM 5SVP 5SVQ 5SVR 5SVT |
Descriptor | P2X purinoceptor 3, 2-acetamido-2-deoxy-beta-D-glucopyranose, MAGNESIUM ION, ... (7 entities in total) |
Functional Keywords | membrane protein, ion channel, anomalous signal mg ion |
Biological source | Homo sapiens (Human) |
Total number of polymer chains | 1 |
Total formula weight | 41713.13 |
Authors | Mansoor, S.E.,Lu, W.,Oosterheert, W.,Shekhar, M.,Tajkhorshid, E.,Gouaux, E. (deposition date: 2016-08-07, release date: 2016-09-28, Last modification date: 2024-10-23) |
Primary citation | Mansoor, S.E.,Lu, W.,Oosterheert, W.,Shekhar, M.,Tajkhorshid, E.,Gouaux, E. X-ray structures define human P2X3 receptor gating cycle and antagonist action. Nature, 538:66-71, 2016 Cited by PubMed Abstract: P2X receptors are trimeric, non-selective cation channels activated by ATP that have important roles in the cardiovascular, neuronal and immune systems. Despite their central function in human physiology and although they are potential targets of therapeutic agents, there are no structures of human P2X receptors. The mechanisms of receptor desensitization and ion permeation, principles of antagonism, and complete structures of the pore-forming transmembrane domains of these receptors remain unclear. Here we report X-ray crystal structures of the human P2X receptor in apo/resting, agonist-bound/open-pore, agonist-bound/closed-pore/desensitized and antagonist-bound/closed states. The open state structure harbours an intracellular motif we term the 'cytoplasmic cap', which stabilizes the open state of the ion channel pore and creates lateral, phospholipid-lined cytoplasmic fenestrations for water and ion egress. The competitive antagonists TNP-ATP and A-317491 stabilize the apo/resting state and reveal the interactions responsible for competitive inhibition. These structures illuminate the conformational rearrangements that underlie P2X receptor gating and provide a foundation for the development of new pharmacological agents. PubMed: 27626375DOI: 10.1038/nature19367 PDB entries with the same primary citation |
Experimental method | X-RAY DIFFRACTION (4.025 Å) |
Structure validation
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