5SVS
Anomalous Mn2+ signal reveals a divalent cation-binding site in the head domain of the ATP-gated human P2X3 ion channel
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-09-20 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.7712 |
Spacegroup name | H 3 2 |
Unit cell lengths | 120.200, 120.200, 236.240 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.775 - 4.025 |
R-factor | 0.2313 |
Rwork | 0.229 |
R-free | 0.27480 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.786 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10_2142: ???)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 4.030 |
Number of reflections | 10534 |
<I/σ(I)> | 11.6 |
Completeness [%] | 99.7 |
Redundancy | 11.34 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277.15 | 25% PEG 400, 100 mM MES, pH 6.85, 50 mM MnCl2 |