2Y83

Actin filament pointed end

Summary for 2Y83

• Entry DOI: 10.2210/pdb2y83/pdb
• Related: 1ALM 1ATN 1EQY 1ESV 1H1V 1IJJ 1J6Z 1KXP 1LCU 1LOT 1M8Q 1MA9 1MVW 1NWK 1O18 1O19 1O1A 1O1B 1O1C 1O1D 1O1E 1O1F 1O1G 1P8Z 1QZ5 1QZ6 1RDW 1RFQ 1RGI 1S22 1SQK 1T44 1UY5 1WUA 1Y64 2A3Z 2A40 2A41 2A42 2A5X 2ASM 2ASO 2ASP 2D1K 2FF3 2FF6 2FXU 2V51 2V52 2VCP 2VYP 2W49 2W4U
• EMDB information: 1872
• Descriptor: ACTIN, ALPHA SKELETAL MUSCLE, ADENOSINE-5'-DIPHOSPHATE, CALCIUM ION (3 entities in total)
• Functional Keywords: contractile protein, cytoskeleton, cell motility
• Biological source: ORYCTOLAGUS CUNICULUS (RABBIT)
• Total number of polymer chains: 6
• Total formula weight: 253979.35

Authors

Narita, A.,Oda, T.,Maeda, Y. (deposition date: 2011-02-02, release date: 2011-03-30, Last modification date: 2024-05-08)

Primary citation

Narita, A.,Oda, T.,Maeda, Y.
Structural Basis for the Slow Dynamics of the Actin Filament Pointed End.
Embo J., 30:1230-, 2011

PubMed Abstract: The actin filament has clear polarity where one end, the pointed end, has a much slower polymerization and depolymerization rate than the other end, the barbed end. This intrinsic difference of the ends significantly affects all actin dynamics in the cell, which has central roles in a wide spectrum of cellular functions. The detailed mechanism underlying this difference has remained elusive, because high-resolution structures of the filament ends have not been available. Here, we present the structure of the actin filament pointed end obtained using a single particle analysis of cryo-electron micrographs. We determined that the terminal pointed end subunit is tilted towards the penultimate subunit, allowing specific and extra loop-to-loop inter-strand contacts between the two end subunits, which is not possible in other parts of the filament. These specific contacts prevent the end subunit from dissociating. For elongation, the loop-to-loop contacts also inhibit the incorporation of another actin monomer at the pointed end. These observations are likely to account for the less dynamic pointed end.

PubMed: 21378753
DOI: 10.1038/EMBOJ.2011.48

Experimental method

ELECTRON MICROSCOPY (22.9 Å)