1IJJ

THE X-RAY CRYSTAL STRUCTURE OF THE COMPLEX BETWEEN RABBIT SKELETAL MUSCLE ACTIN AND LATRUNCULIN A AT 2.85 A RESOLUTION

Summary for 1IJJ

• Entry DOI: 10.2210/pdb1ijj/pdb
• Related: 1ATN 1ESV
• Descriptor: ACTIN, ALPHA SKELETAL MUSCLE, MAGNESIUM ION, ADENOSINE-5'-TRIPHOSPHATE, ... (5 entities in total)
• Functional Keywords: actin, latrunculin, cytoskeleton, contractile protein
• Biological source: Oryctolagus cuniculus (rabbit)
• Cellular location: Cytoplasm, cytoskeleton: P68135
• Total number of polymer chains: 2
• Total formula weight: 86099.98

Authors

Vorobiev, S.M.,Bubb, M.R.,Almo, S.C. (deposition date: 2001-04-26, release date: 2002-04-15, Last modification date: 2023-08-16)

Primary citation

Bubb, M.R.,Govindasamy, L.,Yarmola, E.G.,Vorobiev, S.M.,Almo, S.C.,Somasundaram, T.,Chapman, M.S.,Agbandje-McKenna, M.,McKenna, R.
Polylysine induces an antiparallel actin dimer that nucleates filament assembly: crystal structure at 3.5-A resolution
J.Biol.Chem., 277:20999-21006, 2002

PubMed Abstract: An antiparallel actin dimer has been proposed to be an intermediate species during actin filament nucleation. We now show that latrunculin A, a marine natural product that inhibits actin polymerization, arrests polylysine-induced nucleation at the level of an antiparallel dimer, resulting in its accumulation. These dimers, when composed of pyrene-labeled actin subunits, give rise to a fluorescent excimer, permitting detection during polymerization in vitro. We report the crystallographic structure of the polylysine-actin-latrunculin A complex at 3.5-A resolution. The non-crystallographic contact is consistent with a dimeric structure and confirms the antiparallel orientation of its subunits. The crystallographic contacts reveal that the mobile DNase I binding loop of one subunit of a symmetry-related antiparallel actin dimer is partially stabilized in the interface between the two subunits of a second antiparallel dimer. These results provide a potential explanation for the paradoxical nucleation of actin filaments that have exclusively parallel subunits by a dimer containing antiparallel subunits.

PubMed: 11932258
DOI: 10.1074/jbc.M201371200

Experimental method

X-RAY DIFFRACTION (2.85 Å)