1J6Z

UNCOMPLEXED ACTIN

Summary for 1J6Z

• Entry DOI: 10.2210/pdb1j6z/pdb
• Related: 1ATN 1DB0 1DGA 1EQY 2BTF
• Descriptor: ACTIN ALPHA 1, CALCIUM ION, ADENOSINE-5'-DIPHOSPHATE, ... (5 entities in total)
• Functional Keywords: actin, tetramethylrhodamine-5-maleimide, adp-state, contractile protein
• Biological source: Oryctolagus cuniculus (rabbit)
• Cellular location: Cytoplasm, cytoskeleton: P68135
• Total number of polymer chains: 1
• Total formula weight: 43026.82

Authors

Otterbein, L.R.,Graceffa, P.,Dominguez, R. (deposition date: 2001-05-15, release date: 2001-08-15, Last modification date: 2023-08-16)

Primary citation

Otterbein, L.R.,Graceffa, P.,Dominguez, R.
The crystal structure of uncomplexed actin in the ADP state.
Science, 293:708-711, 2001

PubMed Abstract: The dynamics and polarity of actin filaments are controlled by a conformational change coupled to the hydrolysis of adenosine 5'-triphosphate (ATP) by a mechanism that remains to be elucidated. Actin modified to block polymerization was crystallized in the adenosine 5'-diphosphate (ADP) state, and the structure was solved to 1.54 angstrom resolution. Compared with previous ATP-actin structures from complexes with deoxyribonuclease I, profilin, and gelsolin, monomeric ADP-actin is characterized by a marked conformational change in subdomain 2. The successful crystallization of monomeric actin opens the way to future structure determinations of actin complexes with actin-binding proteins such as myosin.

PubMed: 11474115
DOI: 10.1126/science.1059700

Experimental method

X-RAY DIFFRACTION (1.54 Å)