1RFQ

Actin Crystal Dynamics: Structural Implications for F-actin Nucleation, Polymerization and Branching Mediated by the Anti-parallel Dimer

Summary for 1RFQ

• Entry DOI: 10.2210/pdb1rfq/pdb
• Related: 1RDW
• Descriptor: Actin, alpha skeletal muscle, MAGNESIUM ION, ADENOSINE-5'-TRIPHOSPHATE, ... (5 entities in total)
• Functional Keywords: anti-parallel dimer, polymerization, nucleation, filament, structural protein
• Biological source: Oryctolagus cuniculus (rabbit)
• Cellular location: Cytoplasm, cytoskeleton: P68135
• Total number of polymer chains: 2
• Total formula weight: 85631.30

Authors

Reutzel, R.,Yoshioka, C.,Govindasamy, L.,Yarmola, E.G.,Agbandje-McKenna, M.,Bubb, M.R.,McKenna, R. (deposition date: 2003-11-10, release date: 2003-12-16, Last modification date: 2023-08-23)

Primary citation

Reutzel, R.,Yoshioka, C.,Govindasamy, L.,Yarmola, E.G.,Agbandje-McKenna, M.,Bubb, M.R.,McKenna, R.
Actin crystal dynamics: structural implications for F-actin nucleation, polymerization, and branching mediated by the anti-parallel dimer.
J.Struct.Biol., 146:291-301, 2004

PubMed Abstract: Actin filament nucleation, polymerization, and branching are crucial steps in many forms of cell motility, cell shape, and intracellular organelle movements in a wide range of organisms. Previous biochemical data suggests that an anti-parallel actin dimer can incorporate itself into growing filamentous actin (F-actin) and has a role in branching. Furthermore, it is a widespread belief that nucleation is spawned from an actin trimer complex. Here we present the structures of actin dimers and trimers in two tetragonal crystal systems P4(3)2(1)2 and P4(3). Both crystal systems formed by an induced condensation transformation of a previously reported orthorhombic crystal system P2(1)2(1)2(1). Comparison between the three crystal systems demonstrates the dynamics and flexibility of actin-actin interactions. The dimer and trimer actin rearrangements observed between the three crystal systems may provide insight to in vivo actin-actin interactions that occur during the nucleation, polymerization, and branching of F-actin.

PubMed: 15099571
DOI: 10.1016/j.jsb.2003.12.006

Experimental method

X-RAY DIFFRACTION (3 Å)