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1RFQ

Actin Crystal Dynamics: Structural Implications for F-actin Nucleation, Polymerization and Branching Mediated by the Anti-parallel Dimer

Summary for 1RFQ
Entry DOI10.2210/pdb1rfq/pdb
Related1RDW
DescriptorActin, alpha skeletal muscle, MAGNESIUM ION, ADENOSINE-5'-TRIPHOSPHATE, ... (5 entities in total)
Functional Keywordsanti-parallel dimer, polymerization, nucleation, filament, structural protein
Biological sourceOryctolagus cuniculus (rabbit)
Cellular locationCytoplasm, cytoskeleton: P68135
Total number of polymer chains2
Total formula weight85631.30
Authors
Reutzel, R.,Yoshioka, C.,Govindasamy, L.,Yarmola, E.G.,Agbandje-McKenna, M.,Bubb, M.R.,McKenna, R. (deposition date: 2003-11-10, release date: 2003-12-16, Last modification date: 2023-08-23)
Primary citationReutzel, R.,Yoshioka, C.,Govindasamy, L.,Yarmola, E.G.,Agbandje-McKenna, M.,Bubb, M.R.,McKenna, R.
Actin crystal dynamics: structural implications for F-actin nucleation, polymerization, and branching mediated by the anti-parallel dimer.
J.Struct.Biol., 146:291-301, 2004
Cited by
PubMed Abstract: Actin filament nucleation, polymerization, and branching are crucial steps in many forms of cell motility, cell shape, and intracellular organelle movements in a wide range of organisms. Previous biochemical data suggests that an anti-parallel actin dimer can incorporate itself into growing filamentous actin (F-actin) and has a role in branching. Furthermore, it is a widespread belief that nucleation is spawned from an actin trimer complex. Here we present the structures of actin dimers and trimers in two tetragonal crystal systems P4(3)2(1)2 and P4(3). Both crystal systems formed by an induced condensation transformation of a previously reported orthorhombic crystal system P2(1)2(1)2(1). Comparison between the three crystal systems demonstrates the dynamics and flexibility of actin-actin interactions. The dimer and trimer actin rearrangements observed between the three crystal systems may provide insight to in vivo actin-actin interactions that occur during the nucleation, polymerization, and branching of F-actin.
PubMed: 15099571
DOI: 10.1016/j.jsb.2003.12.006
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (3 Å)
Structure validation

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