2VEN

Structure-based enzyme engineering efforts with an inactive monomeric TIM variant: the importance of a single point mutation for generating an active site with suitable binding properties

Summary for 2VEN

Related1IIH 1KV5 1TPF 1TRD 1TSI 1TTI 1TTJ 2J24 2J27 2V2C 2V2D 2V2H 2VEI 2VEK 1AG1 1DKW 1IIG 1ML1 1MSS 1MTM 1TPD 1TPE 1TRI 2V0T 2V5L 2VEL 4TIM 5TIM 6TIM 2VEM 3TIM
DescriptorGLYCOSOMAL TRIOSEPHOSPHATE ISOMERASE, CITRIC ACID (3 entities in total)
Functional Keywordsisomerase, tim barrel, glycolysis, pentose shunt, gluconeogenesis, lipid synthesis, fatty acid biosynthesis, glycosome
Biological sourceTRYPANOSOMA BRUCEI BRUCEI
Total number of polymer chains2
Total molecular weight51510.7
Authors
Alahuhta, M.,Salin, M.,Casteleijn, M.G.,Kemmer, C.,El-Sayed, I.,Augustyns, K.,Neubauer, P.,Wierenga, R.K. (deposition date: 2007-10-25, release date: 2008-02-19, Last modification date: 2019-07-24)
Primary citation
Alahuhta, M.,Salin, M.,Casteleijn, M.G.,Kemmer, C.,El-Sayed, I.,Augustyns, K.,Neubauer, P.,Wierenga, R.K.
Structure-Based Protein Engineering Efforts with a Monomeric Tim Variant: The Importance of a Single Point Mutation for Generating an Active Site with Suitable Binding Properties.
Protein Eng.Des.Sel., 21:257-, 2008
PubMed: 18239072 (PDB entries with the same primary citation)
DOI: 10.1093/PROTEIN/GZN002
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (2 Å)
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers 0.23960 3.8% 1.9%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution
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171916
PDB entries from 2020-12-02