2IZ9

MS2-RNA HAIRPIN (4ONE -5) COMPLEX

「1DZS」から置き換えられました

2IZ9 の概要

• エントリーDOI: 10.2210/pdb2iz9/pdb
• 関連するPDBエントリー: 1AQ3 1AQ4 1BMS 1MSC 1MST 1MVA 1MVB 1U1Y 1ZDH 1ZDI 1ZDJ 1ZDK 1ZSE 2B2D 2B2E 2B2G 2BNY 2BQ5 2BS0 2BS1 2BU1 2C4Q 2C4Y 2C4Z 2C50 2C51 2IZ8 2IZM 2IZN 2MS2 5MSF 6MSF 7MSF
• 分子名称: MS2 COAT PROTEIN, 5'-R(*AP*CP*AP*UP*GP*AP*GP*GP*AP*U ONEP *AP*CP*CP*CP*AP*UP*GP*U)-3' (3 entities in total)
• 機能のキーワード: virus/rna, hairpin, capsid, levivirus, virus, complex (capsid protein-rna hairpin), virus-rna complex
• 由来する生物種: ENTEROBACTERIO PHAGE MS2; 詳細
• 細胞内の位置: Virion : P03612
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 53306.73

構造登録者

Grahn, E.,Stonehouse, N.J.,Adams, C.J.,Fridborg, K.,Beigelman, L.,Matulic-Adamic, J.,Warriner, S.L.,Stockley, P.G.,Liljas, L. (登録日: 2006-07-25, 公開日: 2006-07-27, 最終更新日: 2024-05-01)

主引用文献

Grahn, E.,Stonehouse, N.J.,Adams, C.J.,Fridborg, K.,Beigelman, L.,Matulic-Adamic, J.,Warriner, S.L.,Stockley, P.G.,Liljas, L.
Deletion of a Single Hydrogen Bonding Atom from the MS2 RNA Operator Leads to Dramatic Rearrangements at the RNA-Coat Protein Interface
Nucleic Acids Res., 28:4611-, 2000

PubMed Abstract: The MS2 coat protein binds specifically to an RNA hairpin formed within the viral genome. By soaking different RNA fragments into crystals of MS2 coat protein capsids it is possible to determine the X-ray structure of the RNA-protein complexes formed. Here we present the structure to 2.85 A resolution of a complex between a chemically modified RNA hairpin variant and the MS2 coat protein. This RNA variant has a substitution at the -5 base position, which has been shown previously to be pyrimidine-specific and is a uracil in the wild-type RNA. The modified RNA hairpin contains a pyridin-4-one base (4one) at this position that lacks the exocyclic 2-oxygen eliminating the possibility of forming a hydrogen bond to asparagine A87 in the protein. The 4one complex structure shows an unprecedented major conformational change in the loop region of the RNA, whereas there is almost no change in the conformation of the protein.

PubMed: 11095669
DOI: 10.1093/NAR/28.23.4611

実験手法

X-RAY DIFFRACTION (2.85 Å)