5MSF

MS2 PROTEIN CAPSID/RNA COMPLEX

Summary for 5MSF

• Entry DOI: 10.2210/pdb5msf/pdb
• Descriptor: 5'-R(*CP*CP*GP*GP*AP*GP*GP*AP*UP*CP*AP*CP*CP*AP*CP*GP*GP*G)-3', MS2 PROTEIN CAPSID (3 entities in total)
• Functional Keywords: capsid protein ms2-rna aptamer complex, rna-protein complex, rna stem loop, bacteriophage ms2, icosahedral virus, virus-rna complex, virus/rna
• Biological source: Enterobacterio phage MS2; More
• Cellular location: Virion (Potential): P03612
• Total number of polymer chains: 5
• Total formula weight: 52866.52

Authors

Rowsell, S.,Stonehouse, N.J.,Convery, M.A.,Adams, C.J.,Ellington, A.D.,Hirao, I.,Peabody, D.S.,Stockley, P.G.,Phillips, S.E.V. (deposition date: 1998-05-15, release date: 1998-11-11, Last modification date: 2023-08-02)

Primary citation

Rowsell, S.,Stonehouse, N.J.,Convery, M.A.,Adams, C.J.,Ellington, A.D.,Hirao, I.,Peabody, D.S.,Stockley, P.G.,Phillips, S.E.
Crystal structures of a series of RNA aptamers complexed to the same protein target.
Nat.Struct.Biol., 5:970-975, 1998

PubMed Abstract: We have determined the crystal structures, at 2.8 A resolution, of two different RNA aptamers, each bound to MS2 coat protein. One of the aptamers contains a non-Watson-Crick base pair, while the other is missing one of the unpaired adenines that make sequence-specific contacts in the wild-type complex. Despite these differences, the RNA aptamers bind in the same location on the protein as the wild-type translational operator. Comparison of these new structures with other MS2-RNA complexes allows us to refine further the definition of the minimal recognition elements and suggests a possible application of the MS2 system for routine structure determination of small nucleic acid motifs.

PubMed: 9808042
DOI: 10.1038/2946

Experimental method

X-RAY DIFFRACTION (2.8 Å)