5MSF
MS2 PROTEIN CAPSID/RNA COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID2 |
| Synchrotron site | ESRF |
| Beamline | ID2 |
| Temperature [K] | 278 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-09-15 |
| Detector | MARRESEARCH |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 287.300, 287.300, 651.600 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.800 |
| R-factor | 0.188 * |
| Rwork | 0.188 |
| R-free | 0.19700 |
| Structure solution method | DIFFERENCE FOURIER |
| Starting model (for MR) | 2ms2 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.380 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | CCP4 |
| Refinement software | X-PLOR (3.860) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.185 | 0.361 |
| Total number of observations | 337020 * | |
| Number of reflections | 172487 | |
| <I/σ(I)> | 3 | 1.9 |
| Completeness [%] | 69.0 | 33.5 * |
| Redundancy | 2 | 1.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.4 | 37 * | Valegard, K., (1997) J.Mol.Biol., 270, 724. * |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 4000 | ||
| 2 | 1 | 1 | NA3PO4 | ||
| 3 | 1 | 1 | NAN3 | ||
| 4 | 1 | 2 | NA3PO4 | ||
| 5 | 1 | 2 | NAN3 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | capsid | 0.6 (mg/ml) | |
| 2 | 1 | drop | PEG8000 | 1.25 (%(w/v)) | |
| 3 | 1 | drop | sodium phosphate | 0.1 (M) | |
| 4 | 1 | drop | sodium azide | 0.02 (%(w/v)) | |
| 5 | 1 | reservoir | sodium phosphate | 0.4 (M) | |
| 6 | 1 | reservoir | sodium azide | 0.02 (%(w/v)) |
