5MSF
MS2 PROTEIN CAPSID/RNA COMPLEX
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID2 |
Synchrotron site | ESRF |
Beamline | ID2 |
Temperature [K] | 278 |
Detector technology | IMAGE PLATE |
Collection date | 1997-09-15 |
Detector | MARRESEARCH |
Spacegroup name | H 3 2 |
Unit cell lengths | 287.300, 287.300, 651.600 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 2.800 |
R-factor | 0.188 * |
Rwork | 0.188 |
R-free | 0.19700 |
Structure solution method | DIFFERENCE FOURIER |
Starting model (for MR) | 2ms2 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.380 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | CCP4 |
Refinement software | X-PLOR (3.860) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.950 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.185 | 0.361 |
Total number of observations | 337020 * | |
Number of reflections | 172487 | |
<I/σ(I)> | 3 | 1.9 |
Completeness [%] | 69.0 | 33.5 * |
Redundancy | 2 | 1.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.4 | 37 * | Valegard, K., (1997) J.Mol.Biol., 270, 724. * |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG 4000 | ||
2 | 1 | 1 | NA3PO4 | ||
3 | 1 | 1 | NAN3 | ||
4 | 1 | 2 | NA3PO4 | ||
5 | 1 | 2 | NAN3 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | capsid | 0.6 (mg/ml) | |
2 | 1 | drop | PEG8000 | 1.25 (%(w/v)) | |
3 | 1 | drop | sodium phosphate | 0.1 (M) | |
4 | 1 | drop | sodium azide | 0.02 (%(w/v)) | |
5 | 1 | reservoir | sodium phosphate | 0.4 (M) | |
6 | 1 | reservoir | sodium azide | 0.02 (%(w/v)) |