1W1G

Crystal Structure of the PDK1 Pleckstrin Homology (PH) domain bound to DiC4-phosphatidylinositol (3,4,5)-trisphosphate

1W1G の概要

• エントリーDOI: 10.2210/pdb1w1g/pdb
• 関連するPDBエントリー: 1H1W 1OKY 1OKZ 1UU3 1UU7 1UU8 1UU9 1UVR 1W1D 1W1H
• 分子名称: 3-PHOSPHOINOSITIDE DEPENDENT PROTEIN KINASE-1, (2R)-3-{[(S)-{[(2S,3R,5S,6S)-2,6-DIHYDROXY-3,4,5-TRIS(PHOSPHONOOXY)CYCLOHEXYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-2-(1-HYDROXY BUTOXY)PROPYL BUTYRATE (3 entities in total)
• 機能のキーワード: transferase, pdk1, phosphoinositide dependent protein kinase 1, pkb, pleckstrin homology domain, inositol phosphate, phosphoinositide, signal transduction, pi3-kinase, pip3 serine/threonine protein kinase
• 由来する生物種: HOMO SAPIENS (HUMAN)
• 細胞内の位置: Cytoplasm: O15530
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 18532.54

構造登録者

Komander, D.,Deak, M.,Alessi, D.R.,Van Aalten, D.M.F. (登録日: 2004-06-21, 公開日: 2004-11-19, 最終更新日: 2026-03-04)

主引用文献

Komander, D.,Fairservice, A.,Deak, M.,Kular, G.S.,Prescott, A.R.,Downes, C.P.,Safrany, S.T.,Alessi, D.R.,Van Aalten, D.M.F.
Structural Insights Into the Regulation of Pdk1 by Phosphoinositides and Inositol Phosphates
Embo J., 23:3918-, 2004

PubMed Abstract: 3-phosphoinositide-dependent protein kinase-1 (PDK1) phosphorylates and activates many kinases belonging to the AGC subfamily. PDK1 possesses a C-terminal pleckstrin homology (PH) domain that interacts with PtdIns(3,4,5)P3/PtdIns(3,4)P2 and with lower affinity to PtdIns(4,5)P2. We describe the crystal structure of the PDK1 PH domain, in the absence and presence of PtdIns(3,4,5)P3 and Ins(1,3,4,5)P4. The structures reveal a 'budded' PH domain fold, possessing an N-terminal extension forming an integral part of the overall fold, and display an unusually spacious ligand-binding site. Mutagenesis and lipid-binding studies were used to define the contribution of residues involved in phosphoinositide binding. Using a novel quantitative binding assay, we found that Ins(1,3,4,5,6)P5 and InsP6, which are present at micromolar levels in the cytosol, interact with full-length PDK1 with nanomolar affinities. Utilising the isolated PDK1 PH domain, which has reduced affinity for Ins(1,3,4,5,6)P5/InsP6, we perform localisation studies that suggest that these inositol phosphates serve to anchor a portion of cellular PDK1 in the cytosol, where it could activate its substrates such as p70 S6-kinase and p90 ribosomal S6 kinase that do not interact with phosphoinositides.

PubMed: 15457207
DOI: 10.1038/SJ.EMBOJ.7600379

実験手法

X-RAY DIFFRACTION (1.45 Å)