1FQV
Insights into scf ubiquitin ligases from the structure of the skp1-skp2 complex
Summary for 1FQV
| Entry DOI | 10.2210/pdb1fqv/pdb |
| Related | 1fs1 1fs2 |
| Descriptor | SKP2, SKP1 (2 entities in total) |
| Functional Keywords | skp1, skp2, f-box, lrr, leucine-rich repeat, scf, ubiquitin, e3, ubiquitin protein ligase, ligase |
| Biological source | Homo sapiens (human) More |
| Total number of polymer chains | 16 |
| Total formula weight | 438214.84 |
| Authors | Schulman, B.A.,Carrano, A.C.,Jeffrey, P.D.,Bowen, Z.,Kinnucan, E.R.,Finnin, M.S.,Elledge, S.J.,Harper, J.W.,Pagano, M.,Pavletich, N.P. (deposition date: 2000-09-06, release date: 2000-11-29, Last modification date: 2024-02-07) |
| Primary citation | Schulman, B.A.,Carrano, A.C.,Jeffrey, P.D.,Bowen, Z.,Kinnucan, E.R.,Finnin, M.S.,Elledge, S.J.,Harper, J.W.,Pagano, M.,Pavletich, N.P. Insights into SCF ubiquitin ligases from the structure of the Skp1-Skp2 complex. Nature, 408:381-386, 2000 Cited by PubMed Abstract: F-box proteins are members of a large family that regulates the cell cycle, the immune response, signalling cascades and developmental programmes by targeting proteins, such as cyclins, cyclin-dependent kinase inhibitors, IkappaBalpha and beta-catenin, for ubiquitination (reviewed in refs 1-3). F-box proteins are the substrate-recognition components of SCF (Skp1-Cullin-F-box protein) ubiquitin-protein ligases. They bind the SCF constant catalytic core by means of the F-box motif interacting with Skp1, and they bind substrates through their variable protein-protein interaction domains. The large number of F-box proteins is thought to allow ubiquitination of numerous, diverse substrates. Most organisms have several Skp1 family members, but the function of these Skp1 homologues and the rules of recognition between different F-box and Skp1 proteins remain unknown. Here we describe the crystal structure of the human F-box protein Skp2 bound to Skp1. Skp1 recruits the F-box protein through a bipartite interface involving both the F-box and the substrate-recognition domain. The structure raises the possibility that different Skp1 family members evolved to function with different subsets of F-box proteins, and suggests that the F-box protein may not only recruit substrate, but may also position it optimally for the ubiquitination reaction. PubMed: 11099048DOI: 10.1038/35042620 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.8 Å) |
Structure validation
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