1FQV

Insights into scf ubiquitin ligases from the structure of the skp1-skp2 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Spacegroup name	C 1 2 1
Unit cell lengths	262.700, 148.200, 133.300
Unit cell angles	90.00, 120.03, 90.00

Refinement procedure

Resolution	8.000 - 2.800
Rwork	0.275
R-free	0.31400
RMSD bond length	0.010 *
RMSD bond angle	1.560 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	8.000
High resolution limit [Å]	2.800
Rmerge	0.074 *	0.194 *
Total number of observations	598299 *
Number of reflections	108372 *
Completeness [%]	93.7 *	93.8 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	4 *	microseeding and macroseeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG4000	15-16 (%)
2	1	reservoir	Tris-HCl	0.1 (M)
3	1	reservoir	ammonium acetate	0.2 (M)
4	1	reservoir	dithiothreitol	10 (mM)