1DX6

STRUCTURE OF ACETYLCHOLINESTERASE COMPLEXED WITH (-)-GALANTHAMINE AT 2.3A RESOLUTION

Summary for 1DX6

• Entry DOI: 10.2210/pdb1dx6/pdb
• Related: 1ACJ 1ACL 1AMN 1AX9 1CFJ 1EVE 1FSS 1VOT 2ACE 2ACK 3ACE 4ACE
• Descriptor: ACETYLCHOLINESTERASE, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, TETRAETHYLENE GLYCOL, ... (6 entities in total)
• Functional Keywords: hydrolase, serine hydrolase, cholinesterase, alzheimer's disease
• Biological source: TORPEDO CALIFORNICA (PACIFIC ELECTRIC RAY)
• Total number of polymer chains: 1
• Total formula weight: 62673.49

Authors

Greenblatt, H.M.,Kryger, G.,Lewis, T.T.,Silman, I.,Sussman, J.L. (deposition date: 1999-12-21, release date: 2000-01-02, Last modification date: 2024-11-20)

Primary citation

Greenblatt, H.M.,Kryger, G.,Lewis, T.T.,Silman, I.,Sussman, J.L.
Structure of Acetylcholinesterase Complexed with (-)-Galanthamine at 2.3A Resolution
FEBS Lett., 463:321-, 1999

PubMed Abstract: (-)-Galanthamine (GAL), an alkaloid from the flower, the common snowdrop (Galanthus nivalis), shows anticholinesterase activity. This property has made GAL the target of research as to its effectiveness in the treatment of Alzheimer's disease. We have solved the X-ray crystal structure of GAL bound in the active site of Torpedo californica acetylcholinesterase (TcAChE) to 2.3 A resolution. The inhibitor binds at the base of the active site gorge of TcAChE, interacting with both the choline-binding site (Trp-84) and the acyl-binding pocket (Phe-288, Phe-290). The tertiary amine group of GAL does not interact closely with Trp-84; rather, the double bond of its cyclohexene ring stacks against the indole ring. The tertiary amine appears to make a non-conventional hydrogen bond, via its N-methyl group, to Asp-72, near the top of the gorge. The hydroxyl group of the inhibitor makes a strong hydrogen bond (2.7 A) with Glu-199. The relatively tight binding of GAL to TcAChE appears to arise from a number of moderate to weak interactions with the protein, coupled to a low entropy cost for binding due to the rigid nature of the inhibitor.

PubMed: 10606746
DOI: 10.1016/S0014-5793(99)01637-3

Experimental method

X-RAY DIFFRACTION (2.3 Å)