1S63

Human protein farnesyltransferase complexed with L-778,123 and FPP

Summary for 1S63

• Entry DOI: 10.2210/pdb1s63/pdb
• Related: 1D8D 1FT1 1LD7 1LD8 1N4Q 1O5M 1S64
• Related PRD ID: PRD_900003
• Descriptor: Protein farnesyltransferase/geranylgeranyltransferase type I alpha subunit, Protein farnesyltransferase beta subunit, beta-D-fructofuranose-(2-1)-alpha-D-glucopyranose, ... (7 entities in total)
• Functional Keywords: farnesyltransferase, ftase, geranylgeranyltransferase, cancer, caax, fti, inhibitor, ras, lipid modification, protein prenylation, transferase
• Biological source: Homo sapiens (human); More
• Total number of polymer chains: 2
• Total formula weight: 94883.16

Authors

Long, S.B.,Casey, P.J.,Beese, L.S. (deposition date: 2004-01-22, release date: 2004-07-27, Last modification date: 2023-08-23)

Primary citation

Reid, T.S.,Long, S.B.,Beese, L.S.
Crystallographic Analysis Reveals that Anticancer Clinical Candidate L-778,123 Inhibits Protein Farnesyltransferase and Geranylgeranyltransferase-I by Different Binding Modes.
Biochemistry, 43:9000-9008, 2004

PubMed Abstract: Many signal transduction proteins that control growth, differentiation, and transformation, including Ras GTPase family members, require the covalent attachment of a lipid group by protein farnesyltransferase (FTase) or protein geranylgeranyltransferase type-I (GGTase-I) for proper function and for the transforming activity of oncogenic mutants. FTase inhibitors are a new class of potential cancer therapeutics under evaluation in human clinical trials. Here, we present crystal structures of the clinical candidate L-778,123 complexed with mammalian FTase and complexed with the related GGTase-I enzyme. Although FTase and GGTase-I have very similar active sites, L-778,123 adopts different binding modes in the two enzymes; in FTase, L-778,123 is competitive with the protein substrate, whereas in GGTase-I, L-778,123 is competitive with the lipid substrate and inhibitor binding is synergized by tetrahedral anions. A comparison of these complexes reveals that small differences in protein structure can dramatically affect inhibitor binding and selectivity. These structures should facilitate the design of more specific inhibitors toward FTase or GGTase-I. Finally, the binding of a drug and anion together could be applicable for developing new classes of inhibitors.

PubMed: 15248757
DOI: 10.1021/bi049280b

Experimental method

X-RAY DIFFRACTION (1.9 Å)