1S63
Human protein farnesyltransferase complexed with L-778,123 and FPP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12B |
Synchrotron site | NSLS |
Beamline | X12B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1999-10-02 |
Detector | ADSC QUANTUM 4 |
Spacegroup name | P 61 |
Unit cell lengths | 178.247, 178.247, 64.518 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 36.670 - 1.900 |
R-factor | 0.161 |
Rwork | 0.161 |
R-free | 0.18400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1jcq |
RMSD bond length | 0.009 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Number of reflections | 88408 | |
<I/σ(I)> | 31.1 | 10.5 |
Completeness [%] | 96.3 | 76 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.7 | 290 | 14% PEG 8000, 400 mM ammonium acetate pH 5.7, 20 mM DTT , VAPOR DIFFUSION, HANGING DROP, temperature 290K, pH 5.70 |