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- PDB-6cw3: Crystal structure of a yeast SAGA transcriptional coactivator Ada... -

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Basic information

Entry
Database: PDB / ID: 6cw3
TitleCrystal structure of a yeast SAGA transcriptional coactivator Ada2/Gcn5 HAT subcomplex, crystal form 2
Components
  • Histone acetyltransferase GCN5
  • Transcriptional adapter 2
  • antibody heavy chain
  • antibody light chain
KeywordsGENE REGULATION / Ada2/Gcn5 structure
Function / homology
Function and homology information


ADA complex / SAGA-type complex / histone crotonyltransferase activity / SLIK (SAGA-like) complex / replication-born double-strand break repair via sister chromatid exchange / histone H3 acetyltransferase activity / rDNA heterochromatin formation / SAGA complex / protein-lysine-acetyltransferase activity / phosphatidylserine binding ...ADA complex / SAGA-type complex / histone crotonyltransferase activity / SLIK (SAGA-like) complex / replication-born double-strand break repair via sister chromatid exchange / histone H3 acetyltransferase activity / rDNA heterochromatin formation / SAGA complex / protein-lysine-acetyltransferase activity / phosphatidylserine binding / histone acetyltransferase activity / Estrogen-dependent gene expression / chromosome, centromeric region / histone acetyltransferase complex / Ub-specific processing proteases / subtelomeric heterochromatin formation / histone H4K16 acetyltransferase activity / histone H3K56 acetyltransferase activity / histone H3K23 acetyltransferase activity / histone H2AK5 acetyltransferase activity / histone H2AK9 acetyltransferase activity / histone H2BK5 acetyltransferase activity / histone H2BK12 acetyltransferase activity / histone H3K4 acetyltransferase activity / histone H3K27 acetyltransferase activity / histone H3K36 acetyltransferase activity / histone H3K122 acetyltransferase activity / histone H3K18 acetyltransferase activity / histone H3K9 acetyltransferase activity / histone H3K14 acetyltransferase activity / histone H4K5 acetyltransferase activity / histone H4K8 acetyltransferase activity / histone H4K12 acetyltransferase activity / histone acetyltransferase / Transferases; Acyltransferases; Transferring groups other than aminoacyl groups / histone reader activity / : / positive regulation of transcription elongation by RNA polymerase II / transcription coregulator activity / chromatin organization / transcription coactivator activity / chromosome, telomeric region / chromatin remodeling / chromatin binding / regulation of transcription by RNA polymerase II / positive regulation of transcription by RNA polymerase II / zinc ion binding / nucleus / cytosol
Similarity search - Function
Transcriptional adaptor 2 / ADA2-like, zinc finger, ZZ-type / : / Transcriptional adapter 2-alpha-like domain / Histone acetyltransferase GCN5 / SWIRM domain / SWIRM domain / SWIRM domain profile. / SANT domain profile. / Zinc finger ZZ-type signature. ...Transcriptional adaptor 2 / ADA2-like, zinc finger, ZZ-type / : / Transcriptional adapter 2-alpha-like domain / Histone acetyltransferase GCN5 / SWIRM domain / SWIRM domain / SWIRM domain profile. / SANT domain profile. / Zinc finger ZZ-type signature. / SANT domain / Zinc-binding domain, present in Dystrophin, CREB-binding protein. / Zinc finger, ZZ type / Zinc finger, ZZ-type / Zinc finger, ZZ-type superfamily / Zinc finger ZZ-type profile. / Myb-like DNA-binding domain / Acetyltransferase (GNAT) family / Gcn5-related N-acetyltransferase (GNAT) domain profile. / SANT SWI3, ADA2, N-CoR and TFIIIB'' DNA-binding domains / SANT/Myb domain / GNAT domain / Acyl-CoA N-acyltransferase / Homeobox-like domain superfamily / Bromodomain, conserved site / Bromodomain signature. / Bromodomain / Bromodomain profile. / bromo domain / Bromodomain / Bromodomain-like superfamily / Winged helix-like DNA-binding domain superfamily / Immunoglobulins / Immunoglobulin-like / Sandwich / Mainly Beta
Similarity search - Domain/homology
Transcriptional adapter 2 / Histone acetyltransferase GCN5
Similarity search - Component
Biological speciesHomo sapiens (human)
Saccharomyces cerevisiae S288c (yeast)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.98 Å
AuthorsSun, J. / Paduch, M. / Kim, S.A. / Kramer, R.M. / Barrios, A.F. / Lu, V. / Luke, J. / Usatyuk, S. / Kossiakoff, A.A. / Tan, S.
Funding support United States, 5items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)GM088236 United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)GM111651 United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)U01GM094588 United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)R01GM072688 United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)U54HG006436 United States
CitationJournal: Proc. Natl. Acad. Sci. U.S.A. / Year: 2018
Title: Structural basis for activation of SAGA histone acetyltransferase Gcn5 by partner subunit Ada2.
Authors: Sun, J. / Paduch, M. / Kim, S.A. / Kramer, R.M. / Barrios, A.F. / Lu, V. / Luke, J. / Usatyuk, S. / Kossiakoff, A.A. / Tan, S.
History
DepositionMar 29, 2018Deposition site: RCSB / Processing site: RCSB
Revision 1.0Sep 19, 2018Provider: repository / Type: Initial release
Revision 1.1Oct 3, 2018Group: Data collection / Database references / Category: citation
Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_ASTM / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year
Revision 1.2Oct 10, 2018Group: Data collection / Database references / Structure summary
Category: citation / entity
Item: _citation.journal_volume / _citation.page_first ..._citation.journal_volume / _citation.page_first / _citation.page_last / _entity.formula_weight
Revision 1.3Jan 1, 2020Group: Author supporting evidence / Structure summary / Category: entity / pdbx_audit_support
Item: _entity.formula_weight / _pdbx_audit_support.funding_organization
Revision 1.4Mar 4, 2020Group: Derived calculations
Category: pdbx_struct_assembly / pdbx_struct_assembly_gen / pdbx_struct_assembly_prop
Revision 1.5Nov 20, 2024Group: Data collection / Database references / Structure summary
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_entry_details / pdbx_modification_feature
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: antibody heavy chain
B: antibody light chain
C: antibody heavy chain
D: antibody light chain
F: Histone acetyltransferase GCN5
E: Transcriptional adapter 2
H: Histone acetyltransferase GCN5
G: Transcriptional adapter 2
hetero molecules


Theoretical massNumber of molelcules
Total (without water)183,54912
Polymers183,2878
Non-polymers2624
Water11,926662
1
A: antibody heavy chain
B: antibody light chain
H: Histone acetyltransferase GCN5
G: Transcriptional adapter 2
hetero molecules


Theoretical massNumber of molelcules
Total (without water)91,7746
Polymers91,6444
Non-polymers1312
Water724
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
2
C: antibody heavy chain
D: antibody light chain
F: Histone acetyltransferase GCN5
E: Transcriptional adapter 2
hetero molecules


Theoretical massNumber of molelcules
Total (without water)91,7746
Polymers91,6444
Non-polymers1312
Water724
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)101.657, 104.061, 166.335
Angle α, β, γ (deg.)90.000, 90.000, 90.000
Int Tables number19
Space group name H-MP212121

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Components

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Protein , 2 types, 4 molecules FHEG

#3: Protein Histone acetyltransferase GCN5


Mass: 29357.150 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae S288c (yeast) / Strain: ATCC 204508 / S288c / Gene: GCN5, ADA4, SWI9, YGR252W / Plasmid: PST44 / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21 (DE3) PLYSS / References: UniProt: Q03330, histone acetyltransferase
#4: Protein Transcriptional adapter 2


Mass: 13858.517 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae S288c (yeast) / Strain: ATCC 204508 / S288c / Gene: ADA2, YDR448W, D9461.33 / Plasmid: PST44 / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21 (DE3) PLYSS / References: UniProt: Q02336

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Antibody , 2 types, 4 molecules ACBD

#1: Antibody antibody heavy chain


Mass: 25088.982 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Production host: Escherichia coli (E. coli)
#2: Antibody antibody light chain


Mass: 23338.916 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Production host: Escherichia coli (E. coli)

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Non-polymers , 2 types, 666 molecules

#5: Chemical
ChemComp-ZN / ZINC ION


Mass: 65.409 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: Zn
#6: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 662 / Source method: isolated from a natural source / Formula: H2O

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Details

Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.41 Å3/Da / Density % sol: 48.93 %
Crystal growTemperature: 294 K / Method: microbatch / pH: 7 / Details: 100 mM succinic acid pH 7.0, 15% w/v PEG3350

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 24-ID-C / Wavelength: 0.9792 Å
DetectorType: DECTRIS PILATUS 6M-F / Detector: PIXEL / Date: Jun 26, 2016
RadiationMonochromator: 0.9792 / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.9792 Å / Relative weight: 1
ReflectionResolution: 1.98→104.06 Å / Num. obs: 123142 / % possible obs: 99.9 % / Redundancy: 6.6 % / Rsym value: 0.062 / Net I/σ(I): 16
Reflection shellResolution: 1.98→2.01 Å / Redundancy: 5.7 % / Num. unique obs: 6024 / Rsym value: 1.465 / % possible all: 99.9

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Processing

Software
NameVersionClassification
PHENIX1.9_1692refinement
PDB_EXTRACT3.24data extraction
XDSdata reduction
Aimlessdata scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT / Resolution: 1.98→88.219 Å / SU ML: 0.27 / Cross valid method: THROUGHOUT / σ(F): 1.37 / Phase error: 23.53 / Stereochemistry target values: ML
RfactorNum. reflection% reflection
Rfree0.2273 6046 4.91 %
Rwork0.2025 116999 -
obs0.2037 123045 99.92 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso max: 206.18 Å2 / Biso mean: 62.9619 Å2 / Biso min: 26.46 Å2
Refinement stepCycle: final / Resolution: 1.98→88.219 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms11171 0 4 662 11837
Biso mean--95.86 58.5 -
Num. residues----1470
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00811428
X-RAY DIFFRACTIONf_angle_d1.15815510
X-RAY DIFFRACTIONf_chiral_restr0.0481744
X-RAY DIFFRACTIONf_plane_restr0.0061968
X-RAY DIFFRACTIONf_dihedral_angle_d14.5124026
LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0 / Total num. of bins used: 30 / % reflection obs: 100 %

Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkNum. reflection all
1.98-2.00250.34452100.313938644074
2.0025-2.02610.32182160.313738304046
2.0261-2.05080.28021930.293738864079
2.0508-2.07670.30921550.279439104065
2.0767-2.10410.29771960.271638414037
2.1041-2.13290.28311920.250738834075
2.1329-2.16340.27862100.241838654075
2.1634-2.19570.26531860.238338214007
2.1957-2.230.24212060.23738714077
2.23-2.26650.25432080.234938934101
2.2665-2.30560.25732240.232938424066
2.3056-2.34750.25482440.233238104054
2.3475-2.39270.26882070.225538364043
2.3927-2.44150.2521900.225939054095
2.4415-2.49460.2691670.228938934060
2.4946-2.55270.26081810.230439354116
2.5527-2.61650.27231810.224738934074
2.6165-2.68730.24322390.222138684107
2.6873-2.76630.26131940.217738844078
2.7663-2.85560.23651750.220339364111
2.8556-2.95770.23821830.222938884071
2.9577-3.07610.25842330.218338874120
3.0761-3.21610.24012030.215439004103
3.2161-3.38570.2192160.208439204136
3.3857-3.59780.21152160.191438854101
3.5978-3.87560.18432030.188539344137
3.8756-4.26560.18862370.161739254162
4.2656-4.88280.15871790.146839954174
4.8828-6.15170.21341950.177640194214
6.1517-88.3070.25582070.216241804387
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
1-0.0078-0.22830.07290.7998-0.35140.2292-0.13850.46290.5114-0.2348-0.4109-0.87240.08120.2603-0.05860.4611-0.02140.04530.7421-0.00210.4458124.901238.3888166.8978
21.5677-1.48820.07681.8431-0.02010.14270.03950.2060.1294-0.21620.0081-0.232-0.17490.17390.00040.3839-0.1034-0.00170.51970.05180.3094119.709345.4264169.3973
30.09960.57320.21960.8749-0.5720.67480.17370.2108-1.3329-0.1641-0.0977-0.66950.95430.0372-0.35050.6321-0.13680.04830.5258-0.23050.4839113.3461-0.113177.8572
42.5175-0.3729-0.44912.0414-1.41820.82870.0910.5574-0.3676-0.31040.0061-0.14710.11590.2147-00.38110.00650.04610.523-0.15680.3789118.05169.0989171.2725
53.87281.57170.25952.3158-0.45092.35620.16790.2775-0.0224-0.0865-0.14660.1516-0.19240.1815-0.00390.3427-0.0264-0.05710.4247-0.01610.265599.079340.3828171.1461
60.6446-0.56990.16111.8379-0.51890.6898-0.03930.0824-0.1911-0.09990.03230.07320.09410.0539-00.3112-0.01140.01570.3494-0.03910.3468108.246317.6204182.8912
70.92180.17760.13831.72650.79682.2142-0.13290.2152-0.21920.11490.02720.02840.15370.0974-0.00330.33220.00310.04140.2877-0.04220.4252108.43038.6252185.8996
81.0210.3025-0.28840.0722-0.22030.1281-0.20590.01530.52750.4366-0.0884-0.2216-0.3930.091700.5782-0.012-0.02390.3356-0.07310.4124115.319748.312208.1522
90.2784-0.3564-0.32112.7171-0.5951.6391-0.09690.10050.20240.36390.03310.0038-0.02820.302500.3795-0.0052-0.06510.3168-0.00590.3013122.842741.5578206.9251
100.628-0.4169-0.66640.31450.20020.32670.14450.26660.3374-0.1233-0.0701-0.61740.06290.1433-00.3758-0.0175-0.0830.34470.04750.4888130.573144.1128200.0107
111.20190.2945-0.89081.3505-0.63870.4490.02670.12520.42670.1112-0.0628-0.1159-0.13290.2752-00.4344-0.063-0.07740.3194-0.01440.4114122.528350.0068203.9478
120.72380.743-0.64141.2136-0.09561.2882-0.1641-0.2864-0.10130.40960.1924-0.14270.07880.0793-00.41610.0211-0.10360.2782-0.00360.269123.238235.038210.6035
131.2098-1.07670.20890.70520.16280.5617-0.007-0.09240.31110.1112-0.12430.2917-0.0666-0.122500.36640.00570.06050.28880.01040.373188.456445.9602198.251
141.5075-0.31221.22052.65730.26670.40980.1285-0.1149-0.01420.2851-0.1590.1175-0.1123-0.011200.3758-0.00850.0140.274-0.03260.259988.836740.3435202.9216
150.06570.49-0.01580.8857-0.86780.54950.0592-0.0981.27590.59330.14520.9747-0.1798-0.35520.03990.44450.00960.12070.4752-0.06110.560980.381145.9567205.4223
160.62760.3820.13150.47130.83370.8808-0.21430.3032-0.022-0.55860.27970.2866-0.01450.083-0.00010.4765-0.0422-0.03970.30950.11670.4435108.683525.2862196.862
172.3341.47491.80553.5629-0.31791.4794-0.14910.1667-0.13390.18250.053-0.1292-0.03750.2645-00.35010.0179-0.01740.28370.03140.2752119.654223.7632205.8574
180.18390.14930.12070.07090.18440.0785-0.08460.4046-0.40990.1526-0.0994-0.3560.1407-0.1578-00.35020.05260.01220.29180.020.4851121.915916.2987202.0887
190.44070.56550.84130.68330.71390.890.022-0.1058-0.28480.71870.0559-0.06680.12960.1719-00.43120.03960.0670.28280.03570.3735111.508421.7587206.3431
200.3075-0.3272-0.09740.39440.09960.2734-0.24-0.009-0.16310.00220.11550.02950.1480.1446-00.3694-0.00810.0220.2890.04260.3117109.999125.2385198.7329
211.2308-0.0467-0.45641.03650.98082.4646-0.04860.2289-0.0108-0.0784-0.14080.1828-0.2301-0.180100.26790.0018-0.01270.3192-0.02030.399786.808135.3543188.8268
221.3142-0.98020.031.03320.11821.43250.03340.1803-0.15460.0547-0.15070.23710.0003-0.109-00.2724-0.0252-0.02920.3234-0.04640.376384.951833.0259188.8396
230.78540.1444-0.38650.47790.54820.04970.05450.33350.435-0.1535-0.068-0.50540.12060.5118-0.00170.4409-0.0021-0.04950.78390.12180.715150.261942.0797199.6943
241.6818-1.44091.75151.1102-1.3041.13180.11680.0647-0.1267-0.0399-0.16440.00170.31080.1539-0.0060.51980.0934-0.04990.56430.08060.5279149.959916.7376218.504
250.884-0.28-0.28790.5011-1.06360.6030.10850.29260.3170.19860.0183-0.4098-0.38670.23780.00070.72770.04120.11120.9010.21170.7402165.84983.7037240.6972
26-0.4057-0.64240.2165-0.1577-0.44930.12770.22380.0978-0.11410.19550.07220.63580.22430.29390.03120.69040.1131-0.16010.72850.17490.7219149.69438.7127226.5349
270.31860.06730.75321.2747-0.02561.54860.49260.0821-0.6736-0.0837-0.2634-0.31780.53490.24340.00190.53450.1563-0.14350.61940.07170.7185144.689713.0896215.8683
283.3384-2.86150.47552.4563-0.40720.2551-1.2672-0.4157-0.58960.11091.8480.0342-0.79171.15380.07871.0283-0.45140.03850.6762-0.14240.8322125.150272.2112172.0293
290.4926-0.4940.12620.29170.0051-0.017-0.2974-0.5014-0.0099-0.30210.12120.1135-0.54370.0703-0.02960.8557-0.24930.0430.7004-0.00160.5191116.908972.6775176.6416
300.72390.8866-0.04190.474-0.03790.36190.2113-0.267-0.3738-0.1423-0.4670.093-0.3803-0.1093-0.02230.756-0.0049-0.04630.4840.0010.3847104.856871.0021172.1221
311.8779-0.08120.25960.70170.12760.03880.13310.06360.1687-0.2649-0.2412-0.3568-0.9981-0.8430.10130.95680.3031-0.02140.74560.0580.4263100.433769.41161.6953
321.05830.4814-0.58690.870.97571.3684-0.2032-0.13420.1539-0.03770.1977-0.11860.205-0.120700.62270.0222-0.22270.4344-0.00210.562574.037381.7563129.8931
332.16540.6377-0.58050.2324-0.21770.25680.1466-1.42371.54040.82060.3822-0.1475-0.3318-0.04840.43780.9323-0.007-0.32370.815-0.29010.697381.626988.6359143.493
340.05630.2450.18980.5894-0.11220.1204-0.3382-0.10150.7567-0.06970.0706-0.2043-0.09330.27600.56450.0177-0.15780.4482-0.11650.73879.231589.9786131.9392
354.07640.6508-0.4430.4976-0.70671.35-0.14990.37250.77281.62760.21670.30.31990.2280.33691.07320.0153-0.1940.3245-0.14010.364781.58376.6838138.2537
362.3473-0.4829-0.78421.93810.1541.12470.0087-0.0325-0.2744-0.1934-0.77060.5285-0.8037-1.5526-1.0771.0130.4303-0.14121.1672-0.08620.517191.954867.2679155.9231
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1chain 'A' and (resid 4 through 20 )A4 - 20
2X-RAY DIFFRACTION2chain 'A' and (resid 21 through 132 )A21 - 132
3X-RAY DIFFRACTION3chain 'A' and (resid 133 through 147 )A133 - 147
4X-RAY DIFFRACTION4chain 'A' and (resid 148 through 227 )A148 - 227
5X-RAY DIFFRACTION5chain 'B' and (resid 2 through 91 )B2 - 91
6X-RAY DIFFRACTION6chain 'B' and (resid 92 through 164 )B92 - 164
7X-RAY DIFFRACTION7chain 'B' and (resid 165 through 214 )B165 - 214
8X-RAY DIFFRACTION8chain 'C' and (resid 4 through 20 )C4 - 20
9X-RAY DIFFRACTION9chain 'C' and (resid 21 through 55 )C21 - 55
10X-RAY DIFFRACTION10chain 'C' and (resid 56 through 70 )C56 - 70
11X-RAY DIFFRACTION11chain 'C' and (resid 71 through 94 )C71 - 94
12X-RAY DIFFRACTION12chain 'C' and (resid 95 through 122 )C95 - 122
13X-RAY DIFFRACTION13chain 'C' and (resid 123 through 158 )C123 - 158
14X-RAY DIFFRACTION14chain 'C' and (resid 159 through 201 )C159 - 201
15X-RAY DIFFRACTION15chain 'C' and (resid 202 through 229 )C202 - 229
16X-RAY DIFFRACTION16chain 'D' and (resid 2 through 19 )D2 - 19
17X-RAY DIFFRACTION17chain 'D' and (resid 20 through 62 )D20 - 62
18X-RAY DIFFRACTION18chain 'D' and (resid 63 through 76 )D63 - 76
19X-RAY DIFFRACTION19chain 'D' and (resid 77 through 91 )D77 - 91
20X-RAY DIFFRACTION20chain 'D' and (resid 92 through 114 )D92 - 114
21X-RAY DIFFRACTION21chain 'D' and (resid 115 through 164 )D115 - 164
22X-RAY DIFFRACTION22chain 'D' and (resid 165 through 214 )D165 - 214
23X-RAY DIFFRACTION23chain 'F' and (resid 98 through 191 )F98 - 191
24X-RAY DIFFRACTION24chain 'F' and (resid 192 through 315 )F192 - 315
25X-RAY DIFFRACTION25chain 'E' and (resid 1 through 49 )E1 - 49
26X-RAY DIFFRACTION26chain 'E' and (resid 50 through 80 )E50 - 80
27X-RAY DIFFRACTION27chain 'E' and (resid 81 through 120 )E81 - 120
28X-RAY DIFFRACTION28chain 'H' and (resid 99 through 127 )H99 - 127
29X-RAY DIFFRACTION29chain 'H' and (resid 128 through 188 )H128 - 188
30X-RAY DIFFRACTION30chain 'H' and (resid 189 through 236 )H189 - 236
31X-RAY DIFFRACTION31chain 'H' and (resid 237 through 279 )H237 - 279
32X-RAY DIFFRACTION32chain 'H' and (resid 280 through 316 )H280 - 316
33X-RAY DIFFRACTION33chain 'G' and (resid 4 through 18 )G4 - 18
34X-RAY DIFFRACTION34chain 'G' and (resid 19 through 49 )G19 - 49
35X-RAY DIFFRACTION35chain 'G' and (resid 50 through 58 )G50 - 58
36X-RAY DIFFRACTION36chain 'G' and (resid 59 through 118 )G59 - 118

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