DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity / DNA topoisomerase (ATP-hydrolysing) / extrinsic component of plasma membrane / DNA topological change / chromosome segregation / chromosome / DNA binding / ATP binding 類似検索 - 分子機能
DNA topoisomerase IV, subunit A, Gram-negative / DNA topoisomerase IV, subunit B, Gram-negative / Rossmann fold - #670 / DNA gyrase/topoisomerase IV, subunit A, C-terminal repeat / DNA gyrase/topoisomerase IV, subunit A, C-terminal / DNA gyrase C-terminal domain, beta-propeller / Histidine kinase/HSP90-like ATPase / DNA gyrase B subunit, C-terminal / DNA gyrase B subunit, carboxyl terminus / DNA topoisomerase, type IIA, alpha-helical domain superfamily ...DNA topoisomerase IV, subunit A, Gram-negative / DNA topoisomerase IV, subunit B, Gram-negative / Rossmann fold - #670 / DNA gyrase/topoisomerase IV, subunit A, C-terminal repeat / DNA gyrase/topoisomerase IV, subunit A, C-terminal / DNA gyrase C-terminal domain, beta-propeller / Histidine kinase/HSP90-like ATPase / DNA gyrase B subunit, C-terminal / DNA gyrase B subunit, carboxyl terminus / DNA topoisomerase, type IIA, alpha-helical domain superfamily / DNA topoisomerase, type IIA, domain A / DNA topoisomerase, type IIA, domain A, alpha-beta / DNA gyrase/topoisomerase IV, subunit A / DNA Topoisomerase IV / DNA topoisomerase, type IIA, subunit B, domain 2 / DNA gyrase B / DNA topoisomerase, type IIA / DNA topoisomerase, type IIA, conserved site / DNA topoisomerase II signature. / TopoisomeraseII / DNA topoisomerase, type IIA, subunit B, C-terminal / Toprim domain / DNA topoisomerase, type IIA-like domain superfamily / Toprim domain profile. / TOPRIM domain / Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase / Histidine kinase-like ATPases / Histidine kinase/HSP90-like ATPase superfamily / Ribosomal protein S5 domain 2-type fold, subgroup / Ribosomal protein S5 domain 2-type fold / Rossmann fold / 3-Layer(aba) Sandwich / Alpha Beta 類似検索 - ドメイン・相同性
Chem-LFX / DNA / DNA (> 10) / DNA topoisomerase 4 subunit A / DNA topoisomerase 4 subunit B 類似検索 - 構成要素
THE SEQUENCES FOR BOTH PARC AND PARE SUBUNITS IN THIS STRUCTURE ARE FROM KLEBSIELLA PNEUMONIAE ...THE SEQUENCES FOR BOTH PARC AND PARE SUBUNITS IN THIS STRUCTURE ARE FROM KLEBSIELLA PNEUMONIAE STRAIN ATCC 35657. THE SUBUNITS ARE CONNECTED BY A TWO RESIDUE LINKER EF. A SIX RESIDUE HIS TAG IS IN THE C-TERMINUS.
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実験情報
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実験
実験
手法: X線回折 / 使用した結晶の数: 1
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試料調製
結晶
マシュー密度: 3.17 Å3/Da / 溶媒含有率: 61.14 %
結晶化
温度: 296 K / 手法: 蒸気拡散法, シッティングドロップ法 / pH: 7.5 詳細: 0.1 M TRIS PH 7.5-8.0, 0-50 MM NACL, 4-8% PEG4000 AND 12-15% GLYCEROL, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 298K PH範囲: 7.5 / Temp details: Incubator