5EIX

QUINOLONE-STABILIZED CLEAVAGE COMPLEX OF TOPOISOMERASE IV FROM KLEBSIELLA PNEUMONIAE

Summary for 5EIX

• Entry DOI: 10.2210/pdb5eix/pdb
• Related: 3LTN 3RAD 3RAE 3RAF 4I3H
• Descriptor: DNA topoisomerase 4 subunit B,DNA topoisomerase 4 subunit A, SYMMETRISED E-SITE (PRE-CUT), MAGNESIUM ION, ... (5 entities in total)
• Functional Keywords: klebsiella pneumoniae, cleavage complex, quinolone, levofloxacin, topoisomerase iv, dna binding, isomerase, isomerase-dna complex, isomerase/dna
• Biological source: Klebsiella pneumoniae; More
• Cellular location: Cell membrane ; Peripheral membrane protein : R4YE07
• Total number of polymer chains: 12
• Total formula weight: 361652.64

Authors

Veselkov, D.A.,Laponogov, I.,Pan, X.-S.,Selvarajah, J.,Branstrom, A.,Fisher, L.M.,Sanderson, M.R. (deposition date: 2015-10-30, release date: 2016-04-13, Last modification date: 2024-05-08)

Primary citation

Veselkov, D.A.,Laponogov, I.,Pan, X.S.,Selvarajah, J.,Skamrova, G.B.,Branstrom, A.,Narasimhan, J.,Prasad, J.V.,Fisher, L.M.,Sanderson, M.R.
Structure of a quinolone-stabilized cleavage complex of topoisomerase IV from Klebsiella pneumoniae and comparison with a related Streptococcus pneumoniae complex.
Acta Crystallogr D Struct Biol, 72:488-496, 2016

PubMed Abstract: Klebsiella pneumoniae is a Gram-negative bacterium that is responsible for a range of common infections, including pulmonary pneumonia, bloodstream infections and meningitis. Certain strains of Klebsiella have become highly resistant to antibiotics. Despite the vast amount of research carried out on this class of bacteria, the molecular structure of its topoisomerase IV, a type II topoisomerase essential for catalysing chromosomal segregation, had remained unknown. In this paper, the structure of its DNA-cleavage complex is reported at 3.35 Å resolution. The complex is comprised of ParC breakage-reunion and ParE TOPRIM domains of K. pneumoniae topoisomerase IV with DNA stabilized by levofloxacin, a broad-spectrum fluoroquinolone antimicrobial agent. This complex is compared with a similar complex from Streptococcus pneumoniae, which has recently been solved.

PubMed: 27050128
DOI: 10.1107/S2059798316001212

Experimental method

X-RAY DIFFRACTION (3.35 Å)